Overview

  • Product nameAnti-SPHK1 antibodySee all SPHK1 primary antibodies ...
  • Description
    Rabbit polyclonal to SPHK1
  • Specificityab46719 reacts with Human SPHK 1 and Murine SPHK 1.
  • Tested applicationsIHC-P, WB, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide: DLESEKYRRLG, corresponding to amino acids 264-274 of Human SPHK1

  • Positive control
    • Human platelet microsomes, Murine kidney, Murine spleen and Raji cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab46719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P Use at an assay dependent dilution.
WB 1/200. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/80.

Target

  • FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
  • Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
  • Sequence similaritiesContains 1 DAGKc domain.
  • Target information above from: UniProt accession Q9NYA1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • SK 1 antibody
    • SK1 antibody
    • Sphingosine Kinase 1 antibody
    • SPHK 1 antibody
    • SPHK antibody
    • Sphk1 antibody
    • SPHK1_HUMAN antibody
    • SPK antibody
    • SPK 1 antibody
    • SPK1 antibody
    see all

Anti-SPHK1 antibody images

  • All lanes : Anti-SPHK1 antibody (ab46719) at 2 µg/ml

    Lane 1 : Human platelet microsomes (20ug)
    Lane 2 : Murine kidney pellet (20ug)
    Lane 3 : Murine spleen supernatant( 40ug)
    Lane 4 : Raji cell lysate (40ug)


    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-SPHK1 antibody (ab46719)

This product has been referenced in:
  • Yamamoto Y  et al. Increased expression of enzymes for sphingosine 1-phosphate turnover and signaling in human decidua during late pregnancy. Biol Reprod 82:628-35 (2010). IHC-P ; Human . Read more (PubMed: 20007411) »
  • Dunlap KA  et al. The sphingosine 1-phosphate (S1P) signaling pathway is regulated during pregnancy in sheep. Biol Reprod 82:876-87 (2010). IHC-P ; Sheep . Read more (PubMed: 20107206) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"