Overview

  • Product nameAnti-SPHK1 antibody
    See all SPHK1 primary antibodies
  • Description
    Rabbit polyclonal to SPHK1
  • Specificityab46719 reacts with Human SPHK 1 and Murine SPHK 1.
  • Tested applicationsIHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog, Cynomolgus Monkey, Rhesus monkey
  • Immunogen

    Synthetic peptide:

    DLESEKYRRLG

    , corresponding to amino acids 264-274 of Human SPHK1

  • Positive control
    • Human platelet microsomes, Murine kidney, Murine spleen and Raji cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab46719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/200. Detects a band of approximately 50 kDa (predicted molecular weight: 43 kDa).
ICC/IF 1/80.

Target

Anti-SPHK1 antibody images

  • All lanes : Anti-SPHK1 antibody (ab46719) at 2 µg/ml

    Lane 1 : Human platelet microsomes (20ug)
    Lane 2 : Murine kidney pellet (20ug)
    Lane 3 : Murine spleen supernatant( 40ug)
    Lane 4 : Raji cell lysate (40ug)


    Predicted band size : 43 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-SPHK1 antibody (ab46719)

This product has been referenced in:
  • Hirata N  et al. Sphingosine-1-phosphate promotes expansion of cancer stem cells via S1PR3 by a ligand-independent Notch activation. Nat Commun 5:4806 (2014). Read more (PubMed: 25254944) »
  • Yamamoto Y  et al. Increased expression of enzymes for sphingosine 1-phosphate turnover and signaling in human decidua during late pregnancy. Biol Reprod 82:628-35 (2010). IHC-P ; Human . Read more (PubMed: 20007411) »

See all 4 Publications for this product

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"