Anti-SPHK1 antibody (ab77475)

Overview

• Product nameAnti-SPHK1 antibodySee all SPHK1 primary antibodies ...
• Description
  Goat polyclonal to SPHK1
• SpecificityThis antibody is expected to recognise both reported isoforms (NP_068807.2; NP_892010.1).
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog, Cynomolgus Monkey
• Immunogen

  Synthetic peptide: DVDLESEKYRRLGE (Human) from the internal region of the protein sequence according to NP_068807.2; NP_892010.1.

  Run BLAST with Run BLAST with
• Positive controlHuman peripheral blood mononucleocyte lysates

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Signaling Pathway
  • Lipid Signaling
  • Lipid Kinases

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • GPCR

Applications

Target

• FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.
• Tissue specificityWidely expressed with highest levels in adult liver, kidney, heart and skeletal muscle.
• Sequence similaritiesContains 1 DAGKc domain.

Target information above from: UniProt accession Q9NYA1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8877 Human
  • Entrez Gene: 8877 Human
  • Entrez Gene: 8877 Human
  • Entrez Gene: 20698 Mouse
  • Entrez Gene: 170897 Rat
  • Omim: 603730 Human
  • SwissProt: Q9NYA1 Human
  • SwissProt: Q9NYA1 Human

  • SwissProt: Q9NYA1 Human
  • SwissProt: Q8CI15 Mouse
  • SwissProt: Q91V26 Rat
  • Unigene: 68061 Human
  • Unigene: 20944 Mouse
  • Unigene: 18522 Rat

  see all

• Alternative names
  SK 1 antibodySK1 antibodySphingosine kinase 1 antibody

  SPHK 1 antibodySPHK antibodySphk1 antibodySPHK1_HUMAN antibodySPK antibodySPK 1 antibody

  see all

Anti-SPHK1 antibody images

• 

  Western blot - SPHK1 antibody (ab77475)
  Anti-SPHK1 antibody (ab77475) at 2 µg/ml + human peripheral blood mononucleocyte lysate in RIPA buffer at 35 µg
  
  Predicted band size : 43 kDa
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)