Overview

  • Product nameAnti-SPHK2 antibodySee all SPHK2 primary antibodies ...
  • Description
    Rabbit polyclonal to SPHK2
  • Tested applicationsIP, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, derived from within residues 600-700 of SPHK2 (Human).

  • Positive control
    • Human cancer tissue, transfected 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 24659784
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 69 kDa.Can be blocked with SPHK2 peptide (ab115809).
IHC-P 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.

Target

  • FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
  • Sequence similaritiesContains 1 DAGKc domain.
  • Cellular localizationCytoplasm. Membrane and Lysosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • SK 2 antibody
    • Sphingosine kinase 2 antibody
    • Sphingosine kinase type 2 antibody
    • SPHK2 antibody
    • SPHK2_HUMAN antibody
    • SPK 2 antibody
    see all

Anti-SPHK2 antibody images

  • ab37977 staining Human breast carcinoma tissue by immunohistochemistry, formalin fixed paraffin embedded tissue, followed by AEC staining.
  • Lane 1 : Anti-SPHK2 antibody (ab37977)
    Lane 2 : c-myc antibody

    Lane 1 : Transfected 293 cell lysate
    Lane 2 : Transfected 293 cell lysate


    Predicted band size : 69 kDa
    Observed band size : 69 kDa
  • Anti-SPHK2 antibody (ab37977) at 1/500 dilution + Mouse NIH 3T3 whole cell lysate at 25 µg

    Secondary
    An HRP-conjugated Donkey Polyclonal at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 69 kDa

    This image is courtesy of an ananymous Abreview

    See Abreview

References for Anti-SPHK2 antibody (ab37977)

This product has been referenced in:
  • Ceccom J  et al. Reduced sphingosine kinase-1 and enhanced sphingosine 1-phosphate lyase expression demonstrate deregulated sphingosine 1-phosphate signaling in Alzheimer's disease. Acta Neuropathol Commun 2:12 (2014). WB ; Human . Read more (PubMed: 24468113) »
  • Novgorodov SA  et al. Essential roles of neutral ceramidase and sphingosine in mitochondrial dysfunction due to traumatic brain injury. J Biol Chem 289:13142-54 (2014). WB, IP ; Mouse . Read more (PubMed: 24659784) »

See all 4 Publications for this product

Product Wall

Thank you for your email.

Yes, I meant His or GST tag etc.

It is more likely that the band appeared in SPHK2 blot is the specific one. However it can be further testified by
- trying no primary control.
- blocking the antibody...

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Thank you for your email.

It is true that the emails are redirected to us if you reply to mailto:technical@abcam.com. I would advise you to log in into your account and check the CCE thread this is the easiest way for tracking the conversation...

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Thank you for your email.

I am sorry I might have missed the information about LDS buffer as cell lysis buffer - indeed there are companies who sells these for cell lysis.

I have requested specific protocol from laboratory I will send...

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Thank you for contacting us.

Just to confirm I have started working on your inquiry so I will get back to you with more information soon.

There is one comment to make here;

ab37977; This SPHK2 antibody is generated from rabb...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Le numéro de commande de remplacement gratuit avec la référenc...

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Thank you for your call earlier this week and for your patience. The blocking peptide of ab37977 is now available on the catalog as ab115809- http://www.abcam.com/SPHK2-peptide-ab115809.html Please let me know if you have any quest...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3)
Loading amount 25 µg
Specification NIH 3T3
Treatment hypoxia
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HMEC endothelial cells)
Loading amount 25 µg
Specification HMEC endothelial cells
Treatment hypoxia
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Feb 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"