Overview

  • Product nameAnti-SPHK2 antibodySee all SPHK2 primary antibodies ...
  • Description
    Rabbit polyclonal to SPHK2
  • Tested applicationsELISA, WB, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, derived from within residues 600-700 of SPHK2 (Human).

  • Positive control
    • Human cancer tissue, transfected 293 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab37977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA
WB
IHC-P
  • Application notesELISA: 1/1000.
    IHC-P: 1/50 - 1/100. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
    WB: 1/100 - 1/500. Detects a band of approximately 69 kDa (predicted molecular weight: 69 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra-and extracellular functions. Also acts on D-erythro-dihydrosphingosine, D-erythro-sphingosine and L-threo-dihydrosphingosine. Binds phosphoinositides.
    • Sequence similaritiesContains 1 DAGKc domain.
    • Cellular localizationCytoplasm. Membrane and Lysosome membrane.
    • Target information above from: UniProt accession Q9NRA0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • SK 2 antibody
      • Sphingosine kinase 2 antibody
      • Sphingosine kinase type 2 antibody
      • SPHK2 antibody
      • SPHK2_HUMAN antibody
      • SPK 2 antibody
      see all

    Anti-SPHK2 antibody images

    • ab37977 staining Human breast carcinoma tissue by immunohistochemistry, formalin fixed paraffin embedded tissue, followed by AEC staining.
    • Lane 1 : Anti-SPHK2 antibody (ab37977)
      Lane 2 : c-myc antibody

      Lane 1 : Transfected 293 cell lysate
      Lane 2 : Transfected 293 cell lysate


      Predicted band size : 69 kDa
      Observed band size : 69 kDa
    • Anti-SPHK2 antibody (ab37977) at 1/500 dilution + Mouse NIH 3T3 whole cell lysate at 25 µg

      Secondary
      An HRP-conjugated Donkey Polyclonal at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 69 kDa

      This image is courtesy of an ananymous Abreview

      See Abreview

    References for Anti-SPHK2 antibody (ab37977)

    This product has been referenced in:
    • Harijith A  et al. Sphingosine Kinase 1 Deficiency Confers Protection against Hyperoxia-Induced Bronchopulmonary Dysplasia in a Murine Model: Role of S1P Signaling and Nox Proteins. Am J Pathol 183:1169-82 (2013). Read more (PubMed: 23933064) »
    • Kim M  et al. Isoflurane activates intestinal sphingosine kinase to protect against bilateral nephrectomy-induced liver and intestine dysfunction. Am J Physiol Renal Physiol 300:F167-76 (2011). WB ; Mouse . Read more (PubMed: 20962114) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your email.

    Yes, I meant His or GST tag etc.

    It is more likely that the band appeared in SPHK2 blot is the specific one. However it can be further testified by
    - trying no primary control.
    - blocking the antibody...

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    Thank you for your email.

    It is true that the emails are redirected to us if you reply to mailto:technical@abcam.com. I would advise you to log in into your account and check the CCE thread this is the easiest way for tracking the conversation...

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    Thank you for your email.

    I am sorry I might have missed the information about LDS buffer as cell lysis buffer - indeed there are companies who sells these for cell lysis.

    I have requested specific protocol from laboratory I will send...

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    Thank you for contacting us.

    Just to confirm I have started working on your inquiry so I will get back to you with more information soon.

    There is one comment to make here;

    ab37977; This SPHK2 antibody is generated from rabb...

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    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

    I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

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    Merci de nous avoir contactés.

    Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas comme attendu. Le numéro de commande de remplacement gratuit avec la référenc...

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    Thank you for your call earlier this week and for your patience. The blocking peptide of ab37977 is now available on the catalog as ab115809- http://www.abcam.com/SPHK2-peptide-ab115809.html Please let me know if you have any quest...

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    Application Western blot
    Sample Mouse Cell lysate - whole cell (NIH 3T3)
    Loading amount 25 µg
    Specification NIH 3T3
    Treatment hypoxia
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2010

    Application Western blot
    Sample Human Cell lysate - whole cell (HMEC endothelial cells)
    Loading amount 25 µg
    Specification HMEC endothelial cells
    Treatment hypoxia
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 22 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"