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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Overview
Product name
Anti-SPIB antibody
Description
Rabbit polyclonal to SPIB
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Pig, Zebrafish
Immunogen
Synthetic peptide: GQQKGNRKRM TYQKLARALR NYAKTGEIRK VKRKLTYQFD SALLPAVRRA , corresponding to amino acids 213-262 of Human SPIB
GQQKGNRKRM TYQKLARALR NYAKTGEIRK VKRKLTYQFD SALLPAVRRA
Positive control
Daudi cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - SPIB antibody (ab42436)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.25 µg/mlPredicted molecular weight: 29 kDa.
ELISA
ELISA: 1/62500ELISA: 1/62500
Target
Function
Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
Tissue specificity
Expressed in plasmacytoid dendritic cells (pDCs) and B-cells, not expressed in T-cells or granulocytes. May also be enriched in stem cell populations of the liver.
Sequence similarities
Belongs to the ETS family.
Contains 1 ETS DNA-binding domain.
Domain
The protein contains a weakly acidic N-terminal transactivation domain (TAD) followed by a second TAD rich in proline, serine and threonine. Each of these domains may be required for transcriptional activation of a subset of target genes.
Cellular localization
Cytoplasm and Nucleus.
Target information above from: UniProt accessionQ01892
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- SPI B antibody
- Spi B transcription factor antibody
- Spib antibody
see all
Database links
- Entrez Gene: 6689 Human
- Entrez Gene: 272382 Mouse
- Entrez Gene: 499146 Rat
- Omim: 606802 Human
- SwissProt: Q01892 Human
- SwissProt: O35905 Mouse
- SwissProt: O35906 Mouse
- SwissProt: Q5EBA3 Rat
see all
Anti-SPIB antibody images:
Western blot - SPIB antibody (ab42436)
All lanes : Anti-SPIB antibody (ab42436) at 0.25 µg/ml
Lane 1 : MW marker
Lane 2 : Daudi cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 29 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
References for Anti-SPIB antibody (ab42436)
ab42436 has not yet been referenced specifically in any publications.
Publishing research using ab42436? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"