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Western blot - SPIB antibody (ab42436)
(enlarge)
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Product Name
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SPIB antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to SPIB
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Immunogen
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Synthetic peptide: GQQKGNRKRM TYQKLARALR NYAKTGEIRK VKRKLTYQFD SALLPAVRRA , corresponding to amino acids 213-262 of Human SPIB
BLAST 'GQQKGNRKRM TYQKLARALR NYAKTGEIRK VKRKLTYQFD SALLPAVRRA ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/62500.
WB: Use at a concentration of 0.25 µg/ml. Predicted molecular weight: 29 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Daudi cell lysate
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
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Relevance
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SPIB is a sequence-specific transcriptional activator that can act as a lymphoid-specific enhancer. SPIB binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3'). Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. May be required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SPIB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for SPIB antibody images, references, products related to ab42436 and other tools.
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SPIB antibody images:
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 Western blot - SPIB antibody (ab42436)
All lanes : SPIB antibody (ab42436) at 0.25 µg/ml
Lane 1 : MW marker
Lane 2 : Daudi cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 29 kDa
Observed band size : 52 kDa (why is the actual band size different from the predicted?)
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Search PubMed (MEDLINE) for references to SPIB
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SPIB antibody - more information
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Customer reviews (feedback) regarding SPIB antibody |
Customer FAQs regarding SPIB antibody |
Protocols for SPIB antibody |
Price and availability of products related to SPIB antibody |
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SPIB antibody - related products:
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SPIB antibody - other tools:
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Contact Abcam with a Technical Enquiry about SPIB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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