Recombinant Anti-SPON2 antibody [EPR9799] (ab171955)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9799] to SPON2
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-SPON2 antibody [EPR9799]
See all SPON2 primary antibodies -
Description
Rabbit monoclonal [EPR9799] to SPON2 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human SPON2 aa 200-300 (Cysteine residue). The exact sequence is proprietary.
Database link: Q9BUD6 -
Positive control
- Fetal lung lysate, A549 lysate, Mouse spleen lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9799 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 36 kDa.
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Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 36 kDa. |
Target
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Function
Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens (By similarity). Binds bacterial lipopolysaccharide (LPS). -
Tissue specificity
Expressed in normal lung tissue but not in lung carcinoma cell lines. -
Sequence similarities
Contains 1 spondin domain.
Contains 1 TSP type-1 domain. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 10417 Human
- Entrez Gene: 100689 Mouse
- Entrez Gene: 171569 Rat
- Omim: 605918 Human
- SwissProt: Q9BUD6 Human
- SwissProt: Q8BMS2 Mouse
- SwissProt: Q9WV75 Rat
- Unigene: 302963 Human
see all -
Alternative names
- Differentially expressed in cancerous and non-cancerous lung cells 1 antibody
- DIL 1 antibody
- DIL-1 antibody
see all
Images
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All lanes : Anti-SPON2 antibody [EPR9799] (ab171955) at 1/1000 dilution
Lane 1 : Fetal lung lysate
Lane 2 : A549 cell lysate
Lane 3 : Mouse spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 36 kDa -
Intracellular flow cytometric analysis of permeabilized A549 labeling Mindin with ab171955 at /100 dilution (red)or a rabbit IgG (negative) (green)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab171955 has been referenced in 3 publications.
- Bai T et al. Deficiency of mindin reduces renal injury after ischemia reperfusion. Mol Med 28:152 (2022). PubMed: 36510147
- Ma HM et al. Overexpression of Spondin-2 Is Associated with Recurrence-Free Survival in Patients with Localized Clear Cell Renal Cell Carcinoma. Dis Markers 2020:5074239 (2020). PubMed: 32952742
- Ni H et al. Spondin-2 is a novel diagnostic biomarker for laryngeal squamous cell carcinoma. Pathol Res Pract N/A:N/A (2018). PubMed: 30527359