Overview

  • Product nameAnti-SPTBN1 antibody [EPR5869]
    See all SPTBN1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5869] to SPTBN1
  • Tested applicationsWB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human SPTBN1.

  • Positive control
    • Human kidney tissue; SH-SY5Y, HeLa, Caco 2 and Jurkat cell lysates.
  • General notesProduced under U.S. Patent No.5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab124888 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 270 kDa (predicted molecular weight: 275 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
    • Tissue specificityIsoform 2 is present in brain, lung and kidney (at protein level).
    • Sequence similaritiesBelongs to the spectrin family.
      Contains 2 CH (calponin-homology) domains.
      Contains 1 PH domain.
      Contains 17 spectrin repeats.
    • Post-translational
      modifications
      Isoform 2 is phosphorylated on Ser-8 and Ser-10.
    • Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta G spectrin antibody
      • Beta II spectrin antibody
      • Beta spectrin 2 antibody
      • Beta spectrin II antibody
      • Beta-II spectrin antibody
      • betaSpII antibody
      • brain 1 antibody
      • ELF antibody
      • Embryonic liver beta fodrin antibody
      • Fodrin beta chain antibody
      • non-erythroid beta chain 1 antibody
      • Spectrin antibody
      • Spectrin beta chain antibody
      • Spectrin, beta, non erythrocytic 1 antibody
      • Spectrin, non erythroid beta chain 1 antibody
      • SPTB2 antibody
      • SPTB2_HUMAN antibody
      • SPTBN1 antibody
      see all

    Anti-SPTBN1 antibody [EPR5869] images

    • ab124888, at 1/250 dilution, staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • All lanes : Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Caco 2 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 275 kDa
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-SPTBN1 antibody [EPR5869] (ab124888)

    ab124888 has not yet been referenced specifically in any publications.

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