Anti-SPTBN1 antibody [EPR5869] (ab124888)

Overview

• Product nameAnti-SPTBN1 antibody [EPR5869]See all SPTBN1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR5869] to SPTBN1
• Tested applicationsWB, IHC-P, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide, corresponding to residues in Human SPTBN1.

• Positive controlHuman kidney tissue; SH-SY5Y, HeLa, Caco 2 and Jurkat cell lysates.
• General notesProduced under U.S. Patent No.5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 4.40 x 10^-11 M
• Storage bufferpH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR5869
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin Crosslinking

  • Tags & Cell Markers
  • Subcellular Markers
  • Plasma Membrane

Applications

Target

• FunctionFodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
• Tissue specificityIsoform 2 is present in brain, lung and kidney (at protein level).
• Sequence similaritiesBelongs to the spectrin family.
  Contains 2 CH (calponin-homology) domains.
  Contains 1 PH domain.
  Contains 17 spectrin repeats.
• Post-translational
  modificationsIsoform 2 is phosphorylated on Ser-8 and Ser-10.
• Cellular localizationCell membrane and Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > M line. Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes.

Target information above from: UniProt accession Q01082 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6711 Human
  • Entrez Gene: 20742 Mouse
  • Entrez Gene: 305614 Rat
  • Omim: 182790 Human
  • SwissProt: Q01082 Human
  • SwissProt: Q62261 Mouse
  • Unigene: 503178 Human
  • Unigene: 705692 Human

  • Unigene: 123110 Mouse
  • Unigene: 466085 Mouse
  • Unigene: 93208 Rat

  see all

• Alternative names
  Beta G spectrin antibodyBeta II spectrin antibodyBeta spectrin 2 antibody

  Beta spectrin II antibodyBeta-II spectrin antibodybetaSpII antibodybrain 1 antibodyELF antibodyEmbryonic liver beta fodrin antibodyFodrin beta chain antibodynon-erythroid beta chain 1 antibodySpectrin antibodySpectrin beta chain antibodySpectrin, beta, non erythrocytic 1 antibodySpectrin, non erythroid beta chain 1 antibodySPTB2 antibodySPTB2_HUMAN antibodySPTBN1 antibody

  see all

Anti-SPTBN1 antibody [EPR5869] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPTBN1 antibody [EPR5869] (ab124888)
  ab124888, at 1/250 dilution, staining SPTBN1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
• 

  Western blot - Anti-SPTBN1 antibody [EPR5869] (ab124888)
  All lanes : Anti-SPTBN1 antibody [EPR5869] (ab124888) at 1/1000 dilution
  
  Lane 1 : SH-SY5Y cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : Caco 2 cell lysate
  Lane 4 : Jurkat cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 275 kDa
  
• 

  Other-Anti-SPTBN1 antibody [EPR5869](ab124888)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D