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Overview

  • Product nameSR1 protein (Tagged-His Tag)See all SR1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 15-183 (169 amino acids) of Human SR1 (Isoform b) with N terminal His tag; MWt 23 kDa (NP_944490.1).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 79 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesQTQDPLYGYFAAVAGQDGQIDADELQRCLTQSGIAGGYKP FNLETCRLM VSMLDRDMSGTMGFNEFKELWAVLNGWRQ HFISFDTDRSGTVDPQELQK ALTTMGFRLSPQAVNSIA KRYSTNGKITFDDYIACCVKLRALTDSFRRR DTAQQGV VNFPYDDFIQCVMSV
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91963 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        22 kDa proteinCalcium binding protein amplified in mutlidrug resistant cellsCP 22
        CP-22CP22SCNSorcinSorcin (class 4 gene)SORCN_HUMANSRIV19
      see all
  • FunctionCalcium-binding protein that modulates excitation-contraction coupling in the heart. Contributes to calcium homeostasis in the heart sarcoplasmic reticulum. Modulates the activity of RYR2 calcium channels.
  • Tissue specificityDetected in cardiac myocytes.
  • Sequence similaritiesContains 4 EF-hand domains.
  • Cellular localizationCytoplasm. Sarcoplasmic reticulum membrane. Relocates to the sarcoplasmic reticulum membrane in response to elevated calcium levels.

    • Target information above from: UniProt accession P30626 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SR1 protein (Tagged-His Tag) images

    • SDS-PAGE - SR1 protein (Tagged-His Tag) (ab91963)
      SDS-PAGE - SR1 protein (Tagged-His Tag) (ab91963)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SR1 protein (Tagged-His Tag) (ab91963)

    ab91963 has not yet been referenced specifically in any publications.

    Publishing research using ab91963 ? Please let us know so that we can cite the reference in this datasheet

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