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ab45062
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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab44153? Please let us know so that we can cite the reference in this datasheet
ab44153 has been referenced in 2 publications.
- Ning J et al. Constitutive Role for IRE1{alpha}-XBP1 Signaling Pathway in the Insulin-Mediated Hepatic Lipogenic Program. Endocrinology 152:2247-55 (2011). PubMed: 21447637
- Piguet AC et al. Hypoxia aggravates non-alcoholic steatohepatitis in mice lacking hepatocellular PTEN. Clin Sci (Lond) 118:401-10 (2010). IHC-P; Mouse. PubMed: 19832698
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SREBP1 antibody (ab44153)
All lanes : Anti-SREBP1 antibody (ab44153) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 120 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
SREBP's are subject to sterol regulated cleavage by site-1 and site-2 proteases which liberate the soluble transcription factor. ab44153 detects a band at 70 kDa, believed to be a cleavage fragment of the full length protein. This detection pattern is consistent with those of other commercially available antibodies.
Immunocytochemistry/ Immunofluorescence - SREBP1 antibody (ab44153)
ICC/IF image of ab44153 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab44153, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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