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ab45062
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Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab44153 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - SREBP1 antibody (ab44153)
All lanes : Anti-SREBP1 antibody (ab44153) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 :
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 120 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
SREBP's are subject to sterol regulated cleavage by site-1 and site-2 proteases which liberate the soluble transcription factor. ab44153 detects a band at 70 kDa, believed to be a cleavage fragment of the full length protein. This detection pattern is consistent with those of other commercially available antibodies.
Immunocytochemistry/ Immunofluorescence - SREBP1 antibody (ab44153)
ICC/IF image of ab44153 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab44153, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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