Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-SREBP1 antibody
See all SREBP1 products (8) ...
Description
Rabbit polyclonal to SREBP1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse
Predicted to work with
Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Mouse SREBP1.
(Peptide available as ab45062.)
Positive control
This antibody gave a positive signal in mouse heart and mouse spleen tissue lysates.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH / Leucine Zipper
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab44153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 70 kDa (predicted molecular weight: 120 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Relevance
SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors which participate in the control of cholesterol homeostasis. SREBP proteins, which are attached to the endoplasmic reticulum and nuclear envelope, are proteolytically cleaved and thus activated in response to conditions of low cellular sterol. Upon activation of SREBP 1 or 2, a 480 - 500 amino acid N-terminal cleavage fragment of these proteins enters the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis. SCA (SREBP cleavage activity) and caspase 3 proteases cleave SREBPs. SREBP proteins containing point mutations at caspase 3 cleavage sites (Asp460 in SREBP 1 and Asp468 in SREBP 2) do not become cleaved following induction of apoptosis, suggesting that SREBPs may play some role in apoptotic processes.
Cellular localization
Cell Membrane, Endoplasmic reticulum, Golgi Apparatus and Nuclear. Multi-pass membrane protein. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Alternative names
- Sterol regulatory element binding protein 1 antibody
- Sterol regulatory element binding protein 1 antibody
- ADD 1 antibody
see all
Database links
- Entrez Gene: 6720 Human
- Entrez Gene: 20787 Mouse
- Entrez Gene: 78968 Rat
- Omim: 184756 Human
- SwissProt: P36956 Human
- SwissProt: Q9WTN3 Mouse
- SwissProt: P56720 Rat
Anti-SREBP1 antibody images:
Western blot - SREBP1 antibody (ab44153)
All lanes : Anti-SREBP1 antibody (ab44153) at 1 µg/ml
Lane 1 : Heart (Mouse) Tissue Lysate
Lane 2 : Spleen (Mouse) Tissue Lysate (ab29293)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 120 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)
SREBP's are subject to sterol regulated cleavage by site-1 and site-2 proteases which liberate the soluble transcription factor. ab44153 detects a band at 70 kDa, believed to be a cleavage fragment of the full length protein. This detection pattern is consistent with those of other commercially available antibodies.
Immunocytochemistry/ Immunofluorescence - SREBP1 antibody (ab44153)
ICC/IF image of ab44153 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab44153, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-SREBP1 antibody (ab44153)
This product has been referenced in:
- Ning Jet al. Constitutive Role for IRE1{alpha}-XBP1 Signaling Pathway in the Insulin-Mediated Hepatic Lipogenic Program. Endocrinology 152:2247-55 (2011).Read more (PubMed: 21447637) »
- Piguet ACet al. Hypoxia aggravates non-alcoholic steatohepatitis in mice lacking hepatocellular PTEN. Clin Sci (Lond) 118:401-10 (2010). IHC-P; Mouse.Read more (PubMed: 19832698) »
See all 2 publications for this product
Publishing research using ab44153? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"