Overview

  • Product nameAnti-SREBP2 antibody
    See all SREBP2 primary antibodies
  • Description
    Rabbit polyclonal to SREBP2
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 300-350 of Human SREBP2 (NP_004590.2).

  • Positive control
    • HeLa and 293T whole cell lysate

Applications

Our Abpromise guarantee covers the use of ab112046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionTranscriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes.
  • Tissue specificityUbiquitously expressed in adult and fetal tissues.
  • Sequence similaritiesBelongs to the SREBP family.
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Post-translational
    modifications
    At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7.
  • Cellular localizationNucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI608257 antibody
    • bHLHd2 antibody
    • Class D basic helix-loop-helix protein 2 antibody
    • OTTHUMP00000028740 antibody
    • Processed sterol regulatory element-binding protein 2 antibody
    • SRBP2_HUMAN antibody
    • SREBF 2 antibody
    • Srebf2 antibody
    • SREBF2 protein antibody
    • SREBP 2 antibody
    • SREBP-2 antibody
    • SREBP2 antibody
    • SREBP2gc antibody
    • sterol regulatory element binding factor 2 antibody
    • Sterol regulatory element binding protein 2 antibody
    • Sterol regulatory element binding transcription factor 2 antibody
    • Sterol regulatory element-binding transcription factor 2 antibody
    see all

Anti-SREBP2 antibody images

  • All lanes : Anti-SREBP2 antibody (ab112046) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 124 kDa


    Exposure time : 3 minutes
  • Detection of SREBP2 by Western Blot of Immunprecipitate.
    Lane 1: ab112046 at 1µg/ml staining SREBP2 in HeLa whole cell lysate immunoprecipitated using ab112046 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References for Anti-SREBP2 antibody (ab112046)

This product has been referenced in:
  • Li X  et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). Read more (PubMed: 24493696) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (prostate cancer cells)
Specification prostate cancer cells
Treatment different concentration of fatostatin for 24hrs
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Xiangyan Li

Verified customer

Submitted Jun 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"