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Overview

  • Product nameSRP14 protein (Tagged-His Tag)See all SRP14 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 3-136 of Human SRP14 with N terminal His tag; 134 amino acids, 26kDa. UniProt ID = P37108
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 68 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium hidrogen phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesLLESEQFLTELTRLFQKCRTSGSVYITLKKYDGRTKPIPK KGTVEGFEPAD NKCLLRATDGKKKISTVVSSKEVNKFQ MAYSNLLRANMDGLKKRDKKNKTK KTKAAAAAAAAAPA AAATAPTTAATTAATAAQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91733 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        18 kDa Alu RNA binding protein18 kDa Alu RNA-binding proteinALURBP
        MGC14326Signal recognition particle 14 kDa proteinSRP 14SRP14SRP14_HUMAN
      see all
  • FunctionSignal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP9 together with SRP14 and the Alu portion of the SRP RNA, constitutes the elongation arrest domain of SRP. The complex of SRP9 and SRP14 is required for SRP RNA binding.
  • Sequence similaritiesBelongs to the SRP14 family.
  • Cellular localizationCytoplasm.

    • Target information above from: UniProt accession P37108 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    SRP14 protein (Tagged-His Tag) images

    • SDS-PAGE - SRP14 protein (Tagged-His Tag) (ab91733)
      SDS-PAGE - SRP14 protein (Tagged-His Tag) (ab91733)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for SRP14 protein (Tagged-His Tag) (ab91733)

    ab91733 has not yet been referenced specifically in any publications.

    Publishing research using ab91733 ? Please let us know so that we can cite the reference in this datasheet

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