Loading...
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Histones >> HMGs
Anti-SRY antibody [SRY.G12]
See all SRY products (3) ...
Mouse monoclonal [SRY.G12] to SRY
ab22166 recognises specifically SRY in XY testes, but not in XX testes.
ELISA, WB, IPmore details
Reacts with
Mouse
Fusion protein (Mouse): SRY
Mouse testes.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
Protein A purified
Monoclonal
SRY.G12
IgG
Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Epigenetics and Nuclear Signaling >> Transcription >> Transcription Factors
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Epigenetics and Nuclear Signaling >> Histones >> HMGs
Western blot - SRY antibody [SRY.G12] (ab22166)
(enlarge)
Our Abpromise guarantee covers the use of ab22166 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/2000 - 1/5000.
WB: 1/1000Detects a band of approximately 39 kDa (predicted molecular weight: 24 kDa).
IP: Use at an assay dependent dilution.
Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity). In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity). Involved in different aspects of gene regulation including promoter activation or repression (By similarity). Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.
Defects in SRY are a cause of gonadal dysgenesis XY female type (GDXY) [MIM:400044]; also known as complete SRY-related 46,XY gonadal dysgenesis or 'XY females' or Swyer syndrome. Patients are found to have a 46,XY karyotype. They suffer rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. In contrast, subjects with 46,XY partial gonadal dysgenesis have ambiguous genitalia, a mix of Muellerian and Wolffian structures, and dysgenic gonads.
Note=A 45,X chromosomal aberration involving SRY is found in Turner syndrome, a disease characterized by gonadal dysgenesis with short stature, "streak gonads", variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female.
Defects in SRY are a cause of true hermaphroditism (TRUHER) [MIM:400045]; also known as complete SRY-positive 46,XX gonadal dysgenesis. A true hermaphrodite must have both mature ovarian and mature testicular tissue with histologic evidence of follicles and tubules, respectively. It is a genetically heterogeneous condition. The genotype of most affected individuals is 46,XX, but many have 46,XY or a mosaic of 46,XX/46,XY. True hermaphroditism can be caused also by chromosomal translocation.
Belongs to the SRY family.
Contains 1 HMG box DNA-binding domain.
DNA binding and bending properties of the HMG domains of human and mouse SRY differ form each other. Human SRY shows more extensive minor groove contacts with DNA and a lower specificity of sequence recognition than mouse SRY.
Phosphorylated on serine residues by PKA. Phosphorylation by PKA enhances its DNA-binding activity and stimulates transcription repression. Acetylation of Lys-136 contributes to its nuclear localization and enhances its interaction with KPNB1. Deacetylated by HDAC3. Poly-ADP-ribosylated by PARP1. ADP-ribosylation reduces its DNA-binding activity.
Nucleus speckle. Cytoplasm. Colocalizes with SOX6 in speckles. Colocalizes with CAML in the nucleus. Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH).
Target information above from: UniProt accession
Q05066
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - SRY antibody [SRY.G12] (ab22166)
![Western blot - SRY antibody [SRY.G12] (ab22166)](/ps/datasheet/Images/22/ab22166/SRY too.jpg)
All lanes : Anti-SRY antibody [SRY.G12] (ab22166)
Lane 1 : Mouse XY testes.
Lane 2 : Mouse mesonephros.
Predicted band size : 24 kDa
Observed band size : 39.5 kDa (why is the actual band size different from the predicted?)
SRY protein was immunoprecipitated from 1 mg total mouse testes (lane 1) or mesonephros (lane 2) tissue with a rabbit anti SRY antibody, followed by western blot analysis using ab22166.
This product has been referenced in:
See all 3 publications for this product
Publishing research using ab22166? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
Find concentration of your lot:
![Western blot - SRY antibody [SRY.G12] (ab22166)](/ps/datasheet/Images/22/ab22166/SRY too.jpg)
All lanes : Anti-SRY antibody [SRY.G12] (ab22166)
Lane 1 : Mouse XY testes.
Lane 2 : Mouse mesonephros.
Predicted band size : 24 kDa
Observed band size : 39.5 kDa (why is the actual band size different from the predicted?)
SRY protein was immunoprecipitated from 1 mg total mouse testes (lane 1) or mesonephros (lane 2) tissue with a rabbit anti SRY antibody, followed by western blot analysis using ab22166.
0
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Call 01223 696 000 or contact us
