SRY antibody [SRY.G12] (ab22166)

Product Name 

SRY antibody [SRY.G12]

Product type 

Primary antibodies

Description 

Mouse monoclonal [SRY.G12] to SRY

Immunogen 

Fusion protein (Mouse): SRY

Reacts with 
(species key)

Ms

Specificity 

ab22166 recognises specifically SRY in XY testes, but not in XX testes.

Tested applications 
(see key)

ELISA, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ELISA: 1/2000 - 1/5000.
IP: Use at an assay dependent dilution.
WB: 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 24 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse testes.

Cellular localization 

Nuclear

Research areas 

Developmental Biology >> Reproduction >> Placental development
Developmental Biology >> Reproduction >> Sex determination
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Chromatin and Nuclear Signaling >> Histones >> HMGs

Relevance 

The Sex Determining Region (SRY) is a gene that encodes a transcription factor that is a member of the high mobility group HMG box family of DNA binding proteins. The gene is located on the short arm of the Y chromosome and has a gene map locus - Yp11.3. Common synonyms of the gene name are Testis Determining Factor (TDF) and Testis Determining Factor on Y (TDY). The main function of the SRY protein is to initiate male sex determination as a transcriptional activator, which regulates a genetic switch in male development. The SRY HMG box recognizes DNA by partial intercalation in the minor groove. The SRY protein then binds to the DNA and distorts its shape (SRY protein in action). In doing so, it regulates genes that control the development of the testes. Transcriptional activator which regulates a genetic switch in male development. It is responsible for initiating male sex determination. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Defects in SRY are a cause of gonadal dysgenesis XY female type (GDXY); also known as 'XY females' or Swyer syndrome. Patients are found to have a 46,XY karyotype. They suffer rapid and early degeneration of their gonads, which are present in the adult as 'streak gonads', consisting mainly of fibrous tissue and variable amounts of ovarian stroma. As a result these patients do not develop secondary sexual characteristics at puberty. The external genitalia in these subjects are completely female, and Muellerian structures are normal. In contrast, subjects with 46,XY partial gonadal dysgenesis have ambiguous genitalia, a mix of Muellerian and Wolffian structures, and dysgenic gonads. Defects in SRY may be a cause of Turner syndrome; also known as Ullrich-Turner syndrome. Turner syndrome has its basis in a 45,X chromosomal aberration. It is characterized by gonadal dysgenesis with short stature, 'streak gonads', variable abnormalities such as webbing of the neck, cubitus valgus, cardiac defects, low posterior hair line. The phenotype is female. Contains 1 HMG box DNA binding domain.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

SRY.G12

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for SRY antibody [SRY.G12] images, references, products related to ab22166 and other tools.

SRY antibody [SRY.G12] images:

References for SRY antibody [SRY.G12] (ab22166)

  This product has been used in: (two most recent references)

Jin VX et al. Identification of an OCT4 and SRY regulatory module using integrated computational and experimental genomics approaches. Genome Res 17:807-17 (2007). PubMed: 17567999

Looyenga BD & Hammer GD Origin and identity of adrenocortical tumors in inhibin knockout mice: implications for cellular plasticity in the adrenal cortex. Mol Endocrinol 20:2848-63 (2006). PubMed: 16873442
If you publish research using ab22166 please let us know so that we can cite the reference on this datasheet.

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