Products:Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab16285 for help.
Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.
|
|||||||||
|
|||||||||
Thank you for your answer The application I will use is the immunofluorescence on PFA fixed cells |
|||||||||
ANSWER: |
Thank you for your quick response. ab119844 has been tested and characterized for ICC/IF so our Abpromise applies for this application. If you need anything further or any help then please let me know. |
||||||||
|
|||||||||
Dear Technical Support I have questions about anti CD15 antibodies. CD15 is synonymous with SSEA-1 but you have to different set of antibodies: CD15 (ab119844 or ab754) and SSEA-1 (ab16285). Do they recognize the same protein? I need Ab against human protein, does the ab16285 react with human? If not may I buy a CD15 antibody to stain the same cells? Which is better for IFr application, the ab119844 or ab754 Thank ou very much |
|||||||||
ANSWER: |
Thank you for your enquiry and your interest in our products. I can confirm that CD15 and SSEA1 are alternative names and these antibodies detect the same protein. Depending when the original antibody was produced and the clone licensed, some antobodies have the CD15 others the SSEA1 as target name. ab754 and ab119844 recognize human protein and both are suitable for immunohistochemistry on formalin-fixed paraffin-embedded (IHC-P) tissue sections. However, ab16285 only detects murine SSEA1. I am not sure what you mean by IFr application? Is it immunofluorescence (ICC/IF) or immunohistochemistry on frozen tissue (IHC-Fr) section? Could you please specify the sample types you wish to use so that I could recommend the most suitable product for you? I hope this helps and if I can assist further, please do not hesitate to contact me. |
||||||||
|
|||||||||
Hello,I need MSDS for the following products ab19857 and ab16285. Can you kindly email them to me.Thank YouRegards, |
|||||||||
ANSWER: |
Thank you very much for your request. I have put the MSDS in the attachment of this email. The MSDS for ab19857 and ab16285 can also be directly downloaded from their datasheet. The link can be found scrolling down, underneath the "storage buffer".
I hope this information is useful. Please do not hesitate to contact us again should you have any additional question. |
||||||||
|
|||||||||
Dear Tech Support Team, Our customer is willing to test ab16285 in IHC-Fr with mouse sections. Are you interested in this type of the information for the Abreview promotion (The customer buys certain antibodies and upload Abreview on Abcam's website and then we give 100% credit note for this antibody to buy next antibody)? Thanks in advance for your assistance and reply. Have a nice week, |
|||||||||
ANSWER: |
DISCOUNT CODE: XXXXXXXXX Expiration date: 19-01-2012
I am very pleased to hear you would like to accept our offer and test ab16285 in IHC-Fr. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IHc-Fr and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
||||||||
|
|||||||||
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.
The laboratory of Peter Andrews
Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).
This image is taken from an anonymous abreview.
6
Call 01223 696 000 or contact us