Anti-SSEA1 antibody [MC480] (ab16285)

  Immunocytochemistry/ Immunofluorescence - SSEA1 antibody [MC480] (ab16285)

ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).

  Immunocytochemistry/ Immunofluorescence - SSEA1 antibody [MC480] (ab16285)

The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285.

The laboratory of Peter Andrews

  Flow Cytometry - SSEA1 antibody [MC480] (ab16285)

Cell surface flow analysis of SSEA1 on D3 mouse ES cells using ab16285 at 1:100 dilution. Purple histogram represents negative control; green line represents anti-SSEA1 antibody (ab16285).

This image is taken from an anonymous abreview.

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