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Immunocytochemistry/ Immunofluorescence - CD15 antibody [MC480] (ab16285)
![Immunocytochemistry/ Immunofluorescence - CD15 antibody [MC480] (ab16285)](/ps/datasheet/images/ap200085-ab16285-081001-1.jpg)
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Immunocytochemistry/ Immunofluorescence - CD15 antibody [MC480] (ab16285)
![Immunocytochemistry/ Immunofluorescence - CD15 antibody [MC480] (ab16285)](/ps/datasheet/images/ab16285_2.jpg)
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Product Name
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CD15 antibody [MC480]
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See all CD15 antibodies (25)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [MC480] to CD15
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Immunogen
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F9 teratocarcinoma stem cells (X-irradiated).
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Reacts with
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Ms, Rat
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Does not react with
(species key)
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Hu
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Specificity
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This antibody reacts with the Stage-specific embryonic antigen-1 (or CD15). This is a carbohydrate epitope on glycolipids and glycoproteins involving fucosylated type 2 blood group.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, IP
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: 1/100.
IHC-Fo: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse embryonic carcinoma cell lines positive for SSEA1 include:
F9, PCC4, ND-1, SCC1, NG2, LT/SV, MH-15, FA-25.
Cell lines negative for SSEA1 include: PYS-2, OTT6050f, B3T3SV. C57SV, K129SV. KCA, QAIB, BW5147.
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Cellular localization
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Golgi apparatus, Golgi stack membrane; Type II membrane protein. Membrane bound form in trans cisternae of Golgi.
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Stem Cells >> Endothelial Progenitors >> Endothelial Markers
Cancer >> Tumor immunology >> CD markers
Stem Cells >> Mesenchymal Stem Cells >> Surface Molecules
Stem Cells >> Hematopoietic Progenitors >> Hematopoietic Stem Cells >> Human Lineage Negative
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Immunology >> Cell Type Markers >> CD >> Adhesion
Tags & Cell Markers >> Cell Type Markers >> Tumor Associated
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Relevance
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The CD15 antigen is expressed on approximately 90% human circulating granulocytes (membranes and granules), 30 to 60% of circulating monocytes and is absent from normal lymphocytes. CD15 antigen is also expressed on Reed Sternberg cells of Hodgkin's disease, on T cell lymphomas including mycosis fungoides and on some leukemias. Outside the hematopoietic system, CD15 expression is described in certain normal and neoplastic epithelial cells and in astrocytes. At least 5 major CD15 antigens (105, 135, 165, 185, 220 kDa) are present on the surface membranes of polymorphonuclear cells. The hapten occurs also in glycolipids. CD15 plays a role in mediating phagocytosis, bactericidal activity and chemotaxis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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MC480
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Myeloma
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P3-x63-Ag8
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Isotype
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IgM
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Tissue culture supernatant was cross flow concentrated and buffer exchanged to PBS
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Form
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Liquid
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Concentration
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2.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Notes
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Isotype is mouse IgM, kappa light chain. From Solter and Knowles, 1978.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this CD15 antibody [MC480] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for CD15 antibody [MC480] images, references, products related to ab16285 and other tools.
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CD15 antibody [MC480] images:
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 Immunocytochemistry/ Immunofluorescence - CD15 antibody [MC480] (ab16285)
ICC/IF image of ab16285 stained mouse embryonic stem cells. The cells were 4% formalin fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgM (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
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Immunocytochemistry/ Immunofluorescence - CD15 antibody [MC480] (ab16285)
The image shows staining of the cell membranes of P19 mouse embryonic carcinoma cells using SSEA1-specific antibody, ab16285. The laboratory of Peter Andrews
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References for CD15 antibody [MC480] (ab16285)
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This product has been used in: (two most recent references)
Mizrak SC et al. Embryonic stem cell-like cells derived from adult human testis. Hum Reprod 25:158-67 (2010). ICC/IF; Human. PubMed: 19815622
Hu G et al. A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal. Genes Dev 23:837-48 (2009). WB; Mouse. PubMed: 19339689
See all 4 publication references for this product.
If you publish research using ab16285 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to CD15
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CD15 antibody [MC480] - more information
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Customer reviews (feedback) regarding CD15 antibody [MC480] |
Customer FAQs regarding CD15 antibody [MC480] |
Protocols for CD15 antibody [MC480] |
Price and availability of products related to CD15 antibody [MC480] |
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CD15 antibody [MC480] - related products:
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CD15 antibody [MC480] - other tools:
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Contact Abcam with a Technical Enquiry about CD15 antibody [MC480] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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