You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker

Description

Mouse monoclonal [MC813] to SSEA4 - Embryonic Stem Cell Marker

Specificity

This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4, a glycolipid carbohydrate epitope) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells.Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Tested applications

ICC/IF, Flow Cyt, IHC-FoFrmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Human embryonal carcinoma cell line 2102Ep.

General notes

The mouse myeloma was fused with a spleen from a Balb/c mouse.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

MC813

Myeloma

Sp2/0

Isotype

IgG3

Light chain type

kappa

Research areas

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Surface Molecules

  • Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287) image (enlarge)

  • Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab16287 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

SSEA-4 (Stage-specific embryonic antigen 4) is a glycoprotein expressed early in embryonic development and in pluripotent stem cells. SSEA-4 can be used as a marker of Human Embryonic Stem Cells, Human Embryonic Carcinoma Cells and Human Embryonic Germ Cells. Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells. Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation.

Cellular localization

Cell Membrane

Alternative names

  • Stage specific embryonic antigen 4 antibody

Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker images:

  Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)

Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)

2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.

  Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)

Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)

anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.

  Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)

Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)

ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor®588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.

This image is a courtesy of Fiona Lewis

See Abreview

References for Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)

This product has been referenced in:

  • Anchan RMet al. Amniocytes can serve a dual function as a source of iPS cells and feeder layers. Hum Mol Genet 20:962-74 (2011). ICC/IF; Human.Read more (PubMed: 21156717) »
  • Bone HKet al. A novel chemically directed route for the generation of definitive endoderm from human embryonic stem cells based on inhibition of GSK-3. J Cell Sci 124:1992-2000 (2011). Flow Cyt; Human.Read more (PubMed: 21610099) »

See all 21 publications for this product

Publishing research using ab16287? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"