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Read our guarantee »Products:Stem Cells >> Embryonic Stem Cells >> Surface Molecules
Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker
Mouse monoclonal [MC813] to SSEA4 - Embryonic Stem Cell Marker
This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4, a glycolipid carbohydrate epitope) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells.Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
ICC/IF, Flow Cyt, IHC-FoFrmore details
Reacts with
Mouse, Human
Human embryonal carcinoma cell line 2102Ep.
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5
Concentration information loading...
IgG fraction
Monoclonal
MC813
Sp2/0
IgG3
kappa
Our Abpromise guarantee covers the use of ab16287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 15 µg/ml
Flow Cyt: Use a concentration of 15 µg/ml
IHC-FoFr: Use at an assay dependent dilution.
SSEA-4 (Stage-specific embryonic antigen 4) is a glycoprotein expressed early in embryonic development and in pluripotent stem cells. SSEA-4 can be used as a marker of Human Embryonic Stem Cells, Human Embryonic Carcinoma Cells and Human Embryonic Germ Cells. Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells. Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation.
Cell Membrane
Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)
![Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)](/ps/datasheet/Images/16/ab16287/ab16287_3.jpg)
2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)
![Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)](/ps/datasheet/Images/16/ab16287/ab16287_6.jpg)
anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)
![Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)](/ps/datasheet/images/16/ab16287/SSEA4-Primary-antibodies-ab16287-4.jpg)
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor®588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.
This image is a courtesy of Fiona Lewis
This product has been referenced in:
See all 21 publications for this product
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![Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)](/ps/datasheet/Images/16/ab16287/ab16287_3.jpg)
2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
![Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)](/ps/datasheet/Images/16/ab16287/ab16287_6.jpg)
anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
![Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)](/ps/datasheet/images/16/ab16287/SSEA4-Primary-antibodies-ab16287-4.jpg)
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor®588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.
This image is a courtesy of Fiona Lewis
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