|
|
|
|
Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)
![Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)](/ps/datasheet/images/ab16287_3.jpg)
(enlarge)
|
|
Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)
![Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)](/ps/datasheet/images/ab16287_6.jpg)
(enlarge)
|
|
Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)
![Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)](/ps/datasheet/images/16/ab16287/SSEA4-Primary-antibodies-ab16287-4.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
SSEA4 antibody [MC813] - Embryonic Stem Cell Marker
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [MC813] to SSEA4 - Embryonic Stem Cell Marker
|
|
Immunogen
|
Human embryonal carcinoma cell line 2102Ep.
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Specificity
|
This antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4, a glycolipid carbohydrate epitope) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells.Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
|
|
Tested applications
(see key)
|
Flow Cyt, ICC/IF, IHC-FoFr
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
Flow Cyt: Use at a concentration of 15 µg/ml.
ICC/IF: Use at a concentration of 15 µg/ml.
IHC-Fo: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cell Membrane
|
|
Research areas
|
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Intracellular
Developmental Biology >> Reproduction >> Germ cell markers
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Stem Cells >> Embryonic Stem Cells >> Surface Molecules
|
|
Relevance
|
SSEA-4 (Stage-specific embryonic antigen 4) is a glycoprotein expressed early in embryonic development and in pluripotent stem cells. SSEA-4 can be used as a marker of Human Embryonic Stem Cells, Human Embryonic Carcinoma Cells and Human Embryonic Germ Cells. Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells. Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation.
|
|
|
|
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
MC813
|
|
Myeloma
|
Sp2/0
|
|
Isotype
|
IgG3
|
|
Light chain type
|
kappa
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: Tris HCl, 500mM Sodium chloride, 200mM Sodium citrate. pH 7.5 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 0.60 - 1.00 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
|
Notes
|
The mouse myeloma was fused with a spleen from a Balb/c mouse.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this SSEA4 antibody [MC813] - Embryonic Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for SSEA4 antibody [MC813] - Embryonic Stem Cell Marker images, references, products related to ab16287 and other tools.
|
| |
SSEA4 antibody [MC813] - Embryonic Stem Cell Marker images:
|
 Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)
2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
|
| |
Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)
anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
|
| |
Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)
ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ immunoflurescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocked with 10% serum for 1 hour. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor®588-conjugated goat polyclonal to mouse IgG was used diluted 1/100 as secondary antibody. This image is a courtesy of Fiona Lewis
See Abreview
|
| |
References for SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)
|
|
This product has been used in: (two most recent references)
Ye L et al. Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases. Proc Natl Acad Sci U S A 106:9826-30 (2009). ICC/IF; Human. PubMed: 19482945
Acharya MM et al. Rescue of radiation-induced cognitive impairment through cranial transplantation of human embryonic stem cells. Proc Natl Acad Sci U S A 106:19150-5 (2009). ICC/IF; Human. PubMed: 19901336
See all 13 publication references for this product.
If you publish research using ab16287 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to SSEA4
|
| |
SSEA4 antibody [MC813] - Embryonic Stem Cell Marker - more information
|
Customer reviews (feedback) regarding SSEA4 antibody [MC813] - Embryonic Stem Cell Marker |
Customer FAQs regarding SSEA4 antibody [MC813] - Embryonic Stem Cell Marker |
Protocols for SSEA4 antibody [MC813] - Embryonic Stem Cell Marker |
Price and availability of products related to SSEA4 antibody [MC813] - Embryonic Stem Cell Marker |
| |
SSEA4 antibody [MC813] - Embryonic Stem Cell Marker - related products:
|
|
|
| |
SSEA4 antibody [MC813] - Embryonic Stem Cell Marker - other tools:
|
Contact Abcam with a Technical Enquiry about SSEA4 antibody [MC813] - Embryonic Stem Cell Marker |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)](/index.html?datasheet=16287#ab16287_3.jpg)
![Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)](/index.html?datasheet=16287#ab16287_6.jpg)
![Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)](/index.html?datasheet=16287#SSEA4-Primary-antibodies-ab16287-4.jpg)
