Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)

Overview

• Product nameAnti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker
• Description
  Mouse monoclonal [MC813] to SSEA4 - Embryonic Stem Cell Marker
• SpecificityThis antibody reacts with the Stage-specific embryonic antigen-4 (SSEA-4, a glycolipid carbohydrate epitope) that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells.Expression of SSEA-4 is down regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.
• Tested applicationsICC/IF, Flow Cyt, IHC-FoFr more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Human embryonal carcinoma cell line 2102Ep.

• General notesThe mouse myeloma was fused with a spleen from a Balb/c mouse.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferpH: 7.50
  Preservative: 0.02% Sodium azide
  Constituents: 5.88% Sodium citrate, Tris HCl, 2.9% Sodium chloride
  
  Some batches of this product contain 0.4M Arginine as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
• Concentration information loading...
• PurityIgG fraction
• Clonality Monoclonal
• Clone numberMC813
• MyelomaSp2/0
• IsotypeIgG3
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Reproduction
  • Germ cell markers

  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Intracellular

  • Stem Cells
  • Germline Stem Cells
  • Embryonic Germ Cells

  • Stem Cells
  • Embryonic Stem Cells
  • Surface Molecules

Applications

Target

• RelevanceSSEA-4 (Stage-specific embryonic antigen 4) is a glycoprotein expressed early in embryonic development and in pluripotent stem cells. SSEA-4 can be used as a marker of Human Embryonic Stem Cells, Human Embryonic Carcinoma Cells and Human Embryonic Germ Cells. Monoclonal antibodies to this target have been widely used in the characterization of pluripotent stem cells. Mouse pluripotent stem cells are not recognised by anti-SSEA-4 antibodies but do express the antigen upon differentiation.
• Cellular localizationCell Membrane

• Alternative names
  Stage specific embryonic antigen 4 antibody

Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker images

• 

  Immunocytochemistry/ Immunofluorescence - SSEA 4 antibody [MC813] (ab16287)
  2102ep Human Embryonic Carcinoma cells were stained with SSEA4 antibody ab16287. As expected, staining localised to the cell surface (green). Nuclei are stained blue using Hoechst.
• 

  Flow Cytometry / FACS - SSEA 4 antibody [MC813] (ab16287)
  anti-SSEA4 antibody, ab16297 can be used as a marker of Embryonic Carcinoma cells in Flow Cytometry/FACS. As can be seen from the histograms, in non-differentiating conditions (i.e. without retinoic acid) NTERA2 cells were recognised by ab16297. However, upon differentiation (addition of retinoic acid), the antibody lost the ability to recognise the cells.
• 

  Immunocytochemistry/ Immunofluorescence - SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)This image is a courtesy of Fiona Lewis

  ab16287 staining SSEA4 in human Embryonic Stem Cells, HUES7 by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton and blocking with 10% serum for 1 hour was performed. Samples were incubated with primary antibody (1/100: in 1% serum, 0.1% Triton in PBS) for 1 hour at 37°C. An Alexa Fluor^®588-conjugated goat polyclonal to mouse IgG was used at dilution at 1/100 as secondary antibody.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - Anti-SSEA4 antibody [MC813] - Embryonic Stem Cell Marker (ab16287)Image from Ferro F et al., PLoS One. 2012;7(7):e41774. Epub 2012 Jul 23. Fig 6.; doi:10.1371/journal.pone.0041774; July 23, 2012, PLoS ONE 7(7): e41774.
  Immunofluorescence analysis of Human dental pulp stem cells, staining SSEA4 with ab16287 at 1/40 dilution. A FITC-conjugated anti-mouse IgG was used as the secondary antibody.