RabMAb

Anti-SSH3BP1 (phospho Y435) antibody [EP3954] (ab109395)

Overview

  • Product nameAnti-SSH3BP1 (phospho Y435) antibody [EP3954]
    See all SSH3BP1 primary antibodies
  • Description
    Rabbit monoclonal [EP3954] to SSH3BP1 (phospho Y435)
  • Specificityab109395 only detects SSH3BP1 phosphorylated at tyrosine 435.
  • Tested applicationsWB, IP, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Human

    Does not react with

    Mouse, Rat
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding tyrosine 435 of Human SSH3BP1.

  • Positive control
    • Jurkat cell lysate treated with pervanadate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab109395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP 1/10 - 1/100.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionMay act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. May play a role in regulation of EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level.
    • Tissue specificityWidely expressed, with highest expression in brain.
    • Involvement in diseaseNote=A chromosomal aberration involving ABI1 is a cause of acute leukemias. Translocation t(10;11)(p11.2;q23) with MLL. ABI1 isoform 2 was found to be present in acute leukemia MLL-ABI1 fusion transcript.
    • Sequence similaritiesBelongs to the ABI family.
      Contains 1 SH3 domain.
      Contains 1 t-SNARE coiled-coil homology domain.
    • DomainThe t-SNARE coiled-coil homology domain is necessary and sufficient for interaction with STX1A.
    • Post-translational
      modifications
      In vitro substrate for v-Abl (By similarity). Phosphorylated on tyrosine residues after serum stimulation or induction by v-Abl.
    • Cellular localizationCytoplasm. Nucleus. Cell projection > lamellipodium. Cell projection > filopodium. Cell projection > growth cone. Cell junction > synapse > synaptosome. Cytoplasm > cytoskeleton. Localized to protruding lamellipodia and filopodia tips. Also localized to neuronal growth cones and synaptosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • Abelson interactor 1 antibody
      • Abi-1 antibody
      • Abi1 antibody
      • ABI1_HUMAN antibody
      • Abl binding protein 4 antibody
      • Abl interactor 1 antibody
      • Abl interactor protein 1 antibody
      • Abl-binding protein 4 antibody
      • AblBP4 antibody
      • e3B1 antibody
      • Eps8 binding protein antibody
      • Eps8 SH3 domain binding protein antibody
      • Eps8 SH3 domain-binding protein antibody
      • Eps8-binding protein antibody
      • Hssh3bp1 antibody
      • Interactor protein AblBP4 antibody
      • NAP1 antibody
      • Nap1 binding protein antibody
      • Nap1-binding protein antibody
      • Nap1BP antibody
      • Spectrin SH3 domain binding protein 1 antibody
      • Spectrin SH3 domain-binding protein 1 antibody
      • SSH3BP antibody
      see all

    Anti-SSH3BP1 (phospho Y435) antibody [EP3954] images

    • All lanes : Anti-SSH3BP1 (phospho Y435) antibody [EP3954] (ab109395) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 55 kDa

    References for Anti-SSH3BP1 (phospho Y435) antibody [EP3954] (ab109395)

    ab109395 has not yet been referenced specifically in any publications.

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