Recombinant Anti-SSPN antibody [EPR15515] (ab186730)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15515] to SSPN
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SSPN antibody [EPR15515]
See all SSPN primary antibodies -
Description
Rabbit monoclonal [EPR15515] to SSPN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart, skeletal muscle, A673 and 293 lysates; Mouse heart, rat brain, rat heart tissue lysates; NIH 3T3 cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15515 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186730 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa). |
Target
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Relevance
The SSPN gene is expressed in a variety of tissues with highest levels in muscle, where alternative splice variants have been observed. In certain tumors KRAS2, SSPN, and ITPR2 are co-amplified. The function of this gene is unknown. -
Cellular localization
Cell membrane, sarcolemma; Multi-pass membrane protein. Note: Also found in myotendinous junctions and in the postsynaptic membrane of neuromuscular junctions. -
Database links
- Entrez Gene: 8082 Human
- Entrez Gene: 16651 Mouse
- Entrez Gene: 500364 Rat
- Omim: 601599 Human
- SwissProt: Q14714 Human
- SwissProt: Q62147 Mouse
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Alternative names
- K ras oncogene associated gene antibody
- K ras oncogene associated protein antibody
- Kirsten ras associated protein antibody
see all
Images
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All lanes : Anti-SSPN antibody [EPR15515] (ab186730) at 1/10000 dilution
Lane 1 : Human fetal heart tissue lysate
Lane 2 : Human skeletal muscle tissue lysate
Lane 3 : A673 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 27 kDa -
All lanes : Anti-SSPN antibody [EPR15515] (ab186730) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat brain tissue lysate
Lane 3 : Rat heart tissue lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 27 kDa
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab186730 has been referenced in 1 publication.
- Perez-Ortiz AC et al. Lack of Delta-Sarcoglycan (Sgcd) Results in Retinal Degeneration. Int J Mol Sci 20:N/A (2019). PubMed: 31689918