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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab26212? Please let us know so that we can cite the reference in this datasheet
ab26212 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - SSRP1 antibody [10D7] (ab26212)](/ps/datasheet/Images/26/ab26212/ab26212.png)
Western blot - SSRP1 antibody [10D7] (ab26212)
Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate
Secondary
Goat anti-mouse Ig conjugated to HRP.
developed using the ECL technique
Predicted band size : 81 kDa
Observed band size : 81 kDa
](/ps/datasheet/images/26/ab26212/SSRP1-Primary-antibodies-ab26212-6.jpg)
Immunocytochemistry/ Immunofluorescence-SSRP1 antibody [10D7](ab26212)
ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
](/ps/datasheet/images/26/ab26212/SSRP1-Primary-antibodies-ab26212-7.jpg)
Flow Cytometry-Anti-SSRP1 antibody [10D7](ab26212)
Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
![SSRP1 antibody [10D7] for Western blot in Mouse (26212)](/ps/datasheet/images/26/ab26212/SSRP1-Primary-antibodies-ab26212-1.jpg)
SSRP1 antibody [10D7] for Western blot in Mouse (26212)
![SSRP1 antibody [10D7] for Immunoprecipitation in Human (26212)](/ps/datasheet/images/26/ab26212/SSRP1-Primary-antibodies-ab26212-5.png)
SSRP1 antibody [10D7] for Immunoprecipitation in Human (26212)
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