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Hi, I am interested in this antibody (ab26212; mouse monoclonal SSRP1). However, can I check if this antibody can recognize mouse SSRP1 protein? There was no information on the sequence of the immunogen. Many thanks. |
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ANSWER: |
Thank you very much for your interest in ab26212. To our knowledge, ab26212 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab26212 now, test it in mouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab26212 in mouse. I will then send a discount code. This code must be issued before purchasing ab26212 so please wait for my reply before ordering. 2. Purchase ab26212 either by phone, fax, or online (www.abcam.com). 3. Test it in mouse. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab26212 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. |
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Sorry I just saw the answer to my question on sequence homology at the end of your email. Yes, I will like to proceed, and will test it on mouse sample. Thanks. |
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ANSWER: |
DISCOUNT CODE: XXXXXXXXX Expiration date: [06-March-2012] I am very pleased to hear you would like to accept our offer and test ab26212 in [Mouse]. This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date. To redeem this offer, please submit an Abreview for [Mouse] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. |
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Hi, I am interested in this antibody (ab26212 mouse monoclonal SSRP1). However, can I check if this antibody can recognize mouse SSRP1 protein? There was no information on the sequence of the immunogen. Many thanks. Regards, Wee Wei |
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ANSWER: |
Thank you very much for your interest in ab26212. We do not currently have an image of ab26212 in mouse. Therefore, I can offer a discount off a future purchase if you buy ab26212 now, use it mouse and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free PRIMARY ANTIBODY. It doesn’t matter whether the result is positive or negative, we would just really like to receive your feedback. Of course, if the results are negative you will be covered by our Abpromise guarantee and eligible for a refund or replacement. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and send an image of ab26212 in mouse. I will then send a discount code. This code must be issued before purchasing ab26212 so please wait for my reply before ordering. 2. Purchase ab2612 either by phone, fax, or online (www.abcam.com). 3. Use it in mouse. 4. Send us an image of the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: http://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount. PS: The mouse and human SSRP1 sequences are almost 97% similar so this antibody will most likely cross react. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-SSRP1 antibody [10D7] (ab26212) at 1 µg/ml + HeLa cell lysate
Secondary
Goat anti-mouse Ig conjugated to HRP.
developed using the ECL technique
Predicted band size : 81 kDa
Observed band size : 81 kDa
ICC/IF image of ab26212 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 10µg/ml) overnight at +4ºC. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing HeLa cells stained with ab26212 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab26212, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
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