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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Anti-SSX6 antibody
Rabbit polyclonal to SSX6
ELISA, WBmore details
Reacts with
Human
Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (IPKIMPEKPA EEGSDSKGVP EASGPQNDGK KLCPPGKASS SEKIHERSGP) of Human SSX6 (NP_775493).
IPKIMPEKPA EEGSDSKGVP EASGPQNDGK KLCPPGKASS SEKIHERSGP
HepG2 cell lysate.
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: 2% Sucrose, PBS
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Immunogen affinity purified
Polyclonal
IgG
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Western blot - SSX6 antibody (ab83385)
(enlarge)
Our Abpromise guarantee covers the use of ab83385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/1562500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
SSX6 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy.
Western blot - SSX6 antibody (ab83385)

Anti-SSX6 antibody (ab83385) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 22 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
ab83385 has not yet been referenced specifically in any publications.
Publishing research using ab83385? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Anti-SSX6 antibody (ab83385) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 22 kDa
Observed band size : 25 kDa (why is the actual band size different from the predicted?)
Gel concentration: 12%
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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