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Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Overview
Product name
Anti-ST14 antibody - Aminoterminal end cleaved protein
See all ST14 products (7) ...
Description
Rabbit polyclonal to ST14 - Aminoterminal end cleaved protein
Specificity
Ab28265 reacts with ST14, and does not recognize matriptase2.
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat
Immunogen
This antibody is made to a synthetic peptide based on the amino end of shed human ST14. (Peptide available as ab41265.)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Matriptase
Cancer >> Invasion/microenvironment >> ECM >> Extracellular matrix >> Other
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: Use at an assay dependent dilution. Predicted molecular weight: 95 kDa. Nonreduced SDS-PAGE Western blots yield bands of 110-95 kDa for ST14 complexed with HAI1 and a 70 kDa band for the single-chain form. The 95 kDa complex resolves to A and B chains of 45 and 25 kDa for ST14, plus a 36 kDa band for the HAI1. The single-chain form is produced by cleavage in the SEA domain, between the stem and the CUB domains and the catalytic domain is liberated by cleavage after the LRLR domains. A recommended starting concentration for Western blots is 1/1,000 when using colorimetric substrates such as BCIP/NBT, and 1/5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Degrades extracellular matrix. Proposed to play a role in breast cancer invasion and metastasis. Exhibits trypsin-like activity as defined by cleavage of synthetic substrates with Arg or Lys as the P1 site.
Involvement in disease
Defects in ST14 are a cause of ichthyosis autosomal recessive with hypotrichosis (ARIH) [MIM:610765]. ARIH is a skin disorder characterized by congenital ichthyosis associated with the presence of less than the normal amount of hair.
Sequence similarities
Belongs to the peptidase S1 family.
Contains 2 CUB domains.
Contains 4 LDL-receptor class A domains.
Contains 1 peptidase S1 domain.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ9Y5Y6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Epithin antibody
- HAI antibody
- Matripase antibody
see all
Database links
- Entrez Gene: 6768 Human
- Entrez Gene: 19143 Mouse
- Entrez Gene: 114093 Rat
- Omim: 606797 Human
- SwissProt: Q9Y5Y6 Human
- SwissProt: P56677 Mouse
- SwissProt: Q543E3 Mouse
- SwissProt: Q9JJI7 Rat
see all
Protocols
References for Anti-ST14 antibody - Aminoterminal end cleaved protein (ab28265)
ab28265 has not yet been referenced specifically in any publications.
Publishing research using ab28265? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"