Products:Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Enzymes >> Other Enzymes
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Customer kindly asked if we can provide any data relating to Matriptase 2 non specificfity. |
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ANSWER: |
Thank you for contacting us.
After having discussion with my lab colleagues, regrettably I have to say we have not tested the cross reactivity of these antibodies with Matriptase 2. We do not have any data for sharing. The matriptase 2 non reactivity comes from lack of homology between the immunogen sequences. I am sorry we will be unable to provide any further detail on this however we can confirm that the antibodies are suitable and guaranteed to detect targets as stated on the datasheet.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ICC/IF image of ab28266 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28266, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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