Overview

  • Product nameAnti-STAM1 antibody
    See all STAM1 primary antibodies
  • Description
    Mouse monoclonal to STAM1
  • Tested applicationsIHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-404 of Human STAM1

Properties

Applications

Our Abpromise guarantee covers the use of ab56346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 3 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionInvolved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. May also play a role in T-cell development. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with HGS (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the STAM family.
    Contains 1 ITAM domain.
    Contains 1 SH3 domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 VHS domain.
  • Post-translational
    modifications
    Phosphorylated on Tyr-198. Phosphorylated in response to IL2, IL3, IL4, IL7, CSF2/GM-CSF, EGF and PDGFB. Phosphorylated by activated PDGFRB.
  • Cellular localizationCytoplasm. Early endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • HSE1 homolog antibody
    • Signal transducing adapter molecule 1 antibody
    • signal transducing adaptor molecule (SH3 domain and ITAM motif) 1 antibody
    • STAM antibody
    • STAM-1 antibody
    • STAM1_HUMAN antibody
    see all

Anti-STAM1 antibody images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human small intestine tissue, staining STAM1 with ab56346 at 3 µg/ml.
  • Western blot against tagged recombinant protein immunogen using ab56346 STAM1 antibody at 1ug/ml. Predicted band size of immunogen is 70 kDa
  • ab56346 staining STAM1 in Human retinal pigmented epithelial cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using Triton X-100 and then incubated with ab56346 at a 1/100 dilution at 25°C. The secondary used was an Alexa-Fluor 488 conjugated donkey polyclonal used at a 1/500 dilution.

    See Abreview

References for Anti-STAM1 antibody (ab56346)

ab56346 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (RPE1 (Retinal Pigmented Epithelial Cell))
Specification RPE1 (Retinal Pigmented Epithelial Cell)
Fixative Paraformaldehyde
Permeabilization Yes - triton X100
Username

Ms. Natacha ZANIN

Verified customer

Submitted Nov 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"