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Anti-STAT2 antibody [Y141] (ab32367)

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Overview

Product name

Anti-STAT2 antibody [Y141]
See all STAT2 products (10) ...

Description

Rabbit monoclonal [Y141] to STAT2

Specificity

This antibody detects both long and short forms of STAT2.

Tested applications

WB, ICC, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

A synthetic peptide corresponding to residues near N-terminus of human Stat-2.

Positive control

K562 cell lysate and human thyroid cancer

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

Y141

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

  • Western blot - STAT2 antibody [Y141] (ab32367)Western blot - STAT2 antibody [Y141] (ab32367) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody [Y141] (ab32367)Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody [Y141] (ab32367) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC: 1/50 - 1/100.
IHC-P: 1/100 - 1/250.
WB: 1/500. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).

Is unsuitable for FACS or IP.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

Post-translational
modifications

Tyrosine phosphorylated in response to IFN-alpha.

Cellular localization

Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Target information above from: UniProt accessionP52630 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Homo sapiens interferon alpha induced transcriptional activator antibody
  • Homo sapiens interferon alpha induced transcriptional activator antibody
  • interferon alpha induced transcriptional activator antibody
  • interferon alpha induced transcriptional activator antibody
  • ISGF3 antibody
  • MGC59816 antibody
  • P113 antibody
  • P113 antibody
  • signal transducer and activator of transcription 2 113kD antibody
  • signal transducer and activator of transcription 2 113kD antibody
  • Signal transducer and activator of transcription 2 antibody
  • STAT113 antibody
  • STAT113 antibody
  • Stat2 antibody
  • STAT2_HUMAN antibody
see all

Database links

see all

Anti-STAT2 antibody [Y141] images:

  Western blot - STAT2 antibody [Y141] (ab32367)

Western blot - STAT2 antibody [Y141] (ab32367)

Anti-STAT2 antibody [Y141] (ab32367) at 1/500 dilution + K562 cell lysate

Predicted band size : 98 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody [Y141] (ab32367)

Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody [Y141] (ab32367)

Immunohistochemical analysis of paraffin-embedded human thyroid cancer using ab32367

References for Anti-STAT2 antibody [Y141] (ab32367)

ab32367 has not yet been referenced specifically in any publications.

Publishing research using ab32367? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"