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Product name
Anti-STAT2 antibody
See all STAT2 products (10) ...
Description
Rabbit polyclonal to STAT2
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide from the C-terminal region of human STAT2.Peptide available as ab107 14.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, 1mg/ml BSA, PBS (Ca2+ and Mg2+ free). pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - STAT2 antibody (ab10624)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000. Predicted molecular weight: 113 kDa.
Target
Function
Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated in response to IFN-alpha.
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
Target information above from: UniProt accessionP52630
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Homo sapiens interferon alpha induced transcriptional activator antibody
- interferon alpha induced transcriptional activator antibody
- ISGF3 antibody
see all
Database links
- Entrez Gene: 6773 Human
- Omim: 600556 Human
- SwissProt: P52630 Human
- Unigene: 530595 Human
Anti-STAT2 antibody images:
Western blot - STAT2 antibody (ab10624)
Predicted band size : 113 kDa
Extracts prepared from HeLa cells were resolved by SDS-PAGE on a 4-20% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4C and incubated with ab10624 for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence.
Protocols
References for Anti-STAT2 antibody (ab10624)
ab10624 has not yet been referenced specifically in any publications.
Publishing research using ab10624? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"