The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 113 kDa.
FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
Sequence similaritiesBelongs to the transcription factor STAT family. Contains 1 SH2 domain.
Post-translational modificationsTyrosine phosphorylated in response to IFN-alpha.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
signal transducer and activator of transcription 2 113kD antibody
Signal transducer and activator of transcription 2 antibody
Anti-STAT2 antibody images
Western blot - STAT2 antibody (ab10624)
Predicted band size : 113 kDa
Extracts prepared from HeLa cells were resolved by SDS-PAGE on a 4-20% polyacrylamide gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4C and incubated with ab10624 for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat F(ab')2 anti-rabbit IgG alkaline phosphatase and the signal was detected by chemiluminescence.