Overview

  • Product nameAnti-STAT2 antibodySee all STAT2 primary antibodies ...
  • Description
    Rabbit polyclonal to STAT2
  • Specificityab53149 detects endogenous levels of total STAT2 protein.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-Y-L-K).

  • Positive control
    • WB: Extracts from HeLa cells IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent dilution.
ELISA 1/5000.

Target

  • FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Target information above from: UniProt accession P52630 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Anti-STAT2 antibody images

  • Immunohistochemical analysis of STAT2 in paraffin embedded human lung carcinoma tissue using ab53149 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • All lanes : Anti-STAT2 antibody (ab53149) at 1/300 dilution

    Lane 1 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with no peptide
    Lane 2 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with peptide


    Predicted band size : 98 kDa

References for Anti-STAT2 antibody (ab53149)

This product has been referenced in:
  • Hill T  et al. IFNgamma mediates DUOX2 expression via a STAT-independent signaling pathway. Biochem Biophys Res Commun 395:270-4 (2010). WB ; Human . Read more (PubMed: 20381453) »

See 1 Publication for this product

Product Wall

Thank you fory our message which has been forwarded to me as my colleague ##### is away from the office today.

I am sorry I am not able to trace any previous correspondence from the email address you have used, and so I hope I can provide ad...

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Thank you for your reply.

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I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the...

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Thank you for your reply and for sending the protocol information. My colleague is out of the office today but I'll be happy to help you with this issue.

To improve the signal, we would recommend using 3% BSA for blocking and up to 1:300 dilu...

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Thank you for you reply. I am sorry to hear that the replacement antibody I had sent is also not giving any signal on WB. Can you please provide a few additional details of your protocol?

1) Is the over-expressed protein human STAT2 or is it ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"