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Anti-STAT2 antibody (ab53149)

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Overview

Product name

Anti-STAT2 antibody
See all STAT2 products (10) ...

Description

Rabbit polyclonal to STAT2

Specificity

ab53149 detects endogenous levels of total STAT2 protein.

Tested applications

WB, IHC-P, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthesized non phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-Y-L-K).

Positive control

WB: Extracts from HeLa cells IHC-P: Human lung carcinoma tissue

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab53149 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

  • Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody (ab53149)Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody (ab53149) image (enlarge)

  • Western blot - STAT2 antibody (ab53149)Western blot - STAT2 antibody (ab53149) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab53149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/300 - 1/1000.Detec...Read more →

    WB: 1/300 - 1/1000.Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Function

Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.

Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

Post-translational
modifications

Tyrosine phosphorylated in response to IFN-alpha.

Cellular localization

Cytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Target information above from: UniProt accessionP52630 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Homo sapiens interferon alpha induced transcriptional activator antibody
  • interferon alpha induced transcriptional activator antibody
  • ISGF3 antibody
  • MGC59816 antibody
  • P113 antibody
  • signal transducer and activator of transcription 2 113kD antibody
  • Signal transducer and activator of transcription 2 antibody
  • STAT113 antibody
  • Stat2 antibody
  • STAT2_HUMAN antibody
see all

Database links

see all

Anti-STAT2 antibody images:

  Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody (ab53149)

Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody (ab53149)

Immunohistochemical analysis of STAT2 in paraffin embedded human lung carcinoma tissue using ab53149 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.

  Western blot - STAT2 antibody (ab53149)

Western blot - STAT2 antibody (ab53149)

All lanes : Anti-STAT2 antibody (ab53149) at 1/300 dilution

Lane 1 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with no peptide
Lane 2 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with peptide


Predicted band size : 98 kDa

References for Anti-STAT2 antibody (ab53149)

This product has been referenced in:

  • Hill Tet al. IFNgamma mediates DUOX2 expression via a STAT-independent signaling pathway. Biochem Biophys Res Commun 395:270-4 (2010). WB; Human.Read more (PubMed: 20381453) »

See 1 publication for this product

Publishing research using ab53149? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"