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Overview

  • Product nameAnti-STAT2 antibodySee all STAT2 primary antibodies ...
  • Description
    Rabbit polyclonal to STAT2
  • Specificityab53149 detects endogenous levels of total STAT2 protein.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-Y-L-K).

  • Positive controlWB: Extracts from HeLa cells IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P IHC-P: Use at an assay dependent dilution.
ELISA ELISA: 1/5000.

Target

  • FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

    • Target information above from: UniProt accession P52630 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      Homo sapiens interferon alpha induced transcriptional activator antibodyinterferon alpha induced transcriptional activator antibodyISGF3 antibody
      MGC59816 antibodyP113 antibodysignal transducer and activator of transcription 2 113kD antibodySignal transducer and activator of transcription 2 antibodySTAT113 antibodyStat2 antibodySTAT2_HUMAN antibody
    see all

Anti-STAT2 antibody images

  • Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody (ab53149)
    Immunohistochemistry (Paraffin-embedded sections) - STAT2 antibody (ab53149)
    Immunohistochemical analysis of STAT2 in paraffin embedded human lung carcinoma tissue using ab53149 at a dilution of 1/50. Right hand image: treated with immunising peptide. Left hand image: untreated.
  • Western blot - STAT2 antibody (ab53149)
    Western blot - STAT2 antibody (ab53149)
    All lanes : Anti-STAT2 antibody (ab53149) at 1/300 dilution

    Lane 1 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with no peptide
    Lane 2 : Extracts from HeLa cell treated with IFN (2500U/ml, 30 min) with peptide


    Predicted band size : 98 kDa

References for Anti-STAT2 antibody (ab53149)

This product has been referenced in:
  • Hill T  et al. IFNgamma mediates DUOX2 expression via a STAT-independent signaling pathway. Biochem Biophys Res Commun 395:270-4 (2010). WB ; Human . Read more (PubMed: 20381453) »

See 1 Publication for this product

Publishing research using ab53149 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

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Displaying 1 - 4 of 4 results for Abreviews and Q&A

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Below are the antibodies used before. As we're staining 153 liver biopsy slides we'd ideally like to confirm the efficacy ourselves with a tester pack. I'll get back to you on the quantity. Just to confirm for staining slides, you mentioned e.g. STAT1 (ab...

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Thank you fory our message which has been forwarded to me as my colleague ##### is away from the office today.

I am sorry I am not able to trace any previous correspondence from the email address you have used, and so I hope I can provide adequ...

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I have tried it with 1:300 and the signal was very very faint with ˜1 hr exposure. It would be great if we would can get a credit for this antibody.

Thanks,

Thank you for your reply.

Your credit note ID is ***

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the in...

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I am replying to your email on behalf of Dr. Saito as I was the one who performed the experiment.

1) Yes, the overexpressed STAT2 is human. Initially, I trypsinized the cells to dissociate them. Then, I added media to inactivate the trypsin. I ...

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Thank you for your reply and for sending the protocol information. My colleague is out of the office today but I'll be happy to help you with this issue.

To improve the signal, we would recommend using 3% BSA for blocking and up to 1:300 dilutio...

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First off, thank you for sending the STAT2 Antibody so promptly. However, I have tested it out and it does not seem to be working very well. I have overexpressed STAT2 in Huh7, a hepatocarcinoma cell line, and unfortunately the Ab did not detect it.
...

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Thank you for you reply. I am sorry to hear that the replacement antibody I had sent is also not giving any signal on WB. Can you please provide a few additional details of your protocol?

1) Is the over-expressed protein human STAT2 or is it fro...

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