Anti-STAT2 antibody (ab93445)

Overview

• Product nameAnti-STAT2 antibodySee all STAT2 primary antibodies ...
• Description
  Rabbit polyclonal to STAT2
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Rat
  Predicted to work with: Cow, Human, Pig
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Mouse STAT2.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in the following tissue lysates: Mouse heart, Mouse Spleen, Rat Lung, Rat Heart.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • STATs

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • STATs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
• Sequence similaritiesBelongs to the transcription factor STAT family.
  Contains 1 SH2 domain.
• Post-translational
  modificationsTyrosine phosphorylated in response to IFN-alpha.
• Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Target information above from: UniProt accession P52630 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6773 Human
  • Entrez Gene: 20847 Mouse
  • Entrez Gene: 396923 Pig
  • Entrez Gene: 288774 Rat
  • Omim: 600556 Human
  • SwissProt: P52630 Human
  • SwissProt: Q9WVL2 Mouse
  • SwissProt: O02799 Pig

  • Unigene: 530595 Human
  • Unigene: 293120 Mouse
  • Unigene: 471333 Mouse
  • Unigene: 24237 Rat

  see all

• Alternative names
  Homo sapiens interferon alpha induced transcriptional activator antibodyinterferon alpha induced transcriptional activator antibodyISGF3 antibody

  MGC59816 antibodyP113 antibodysignal transducer and activator of transcription 2 113kD antibodySignal transducer and activator of transcription 2 antibodySTAT113 antibodyStat2 antibodySTAT2_HUMAN antibody

  see all

Anti-STAT2 antibody images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-STAT2 antibody (ab93445)

  ICC/IF image of ab93445 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab93445 at 10µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Western blot - Anti-STAT2 antibody (ab93445)
  All lanes : Anti-STAT2 antibody (ab93445) at 1 µg/ml
  
  Lane 1 : Heart (Mouse) Tissue Lysate
  Lane 2 : Spleen (Mouse) Tissue Lysate
  Lane 3 : Lung (Rat) Tissue Lysate
  Lane 4 : Heart (Rat) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 105 kDa
  Observed band size : 105 kDa
  Additional bands at : 10 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes