Anti-STAT2 (phospho Y690) antibody (ab53132)

Overview

• Product nameAnti-STAT2 (phospho Y690) antibodySee all STAT2 primary antibodies ...
• Description
  Rabbit polyclonal to STAT2 (phospho Y690)
• Specificityab53132 detects endogenous levels of STAT2 only when phosphorylated at tyrosine 690.
• Tested applicationsWB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-Y^P-L-K).

• Positive controlWB: HeLa cell extracts IHC-P: Human lung carcinoma tissue

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab53132 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • STATs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • STATs

Applications

Target

• FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
• Sequence similaritiesBelongs to the transcription factor STAT family.
  Contains 1 SH2 domain.
• Post-translational
  modificationsTyrosine phosphorylated in response to IFN-alpha.
• Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.

Target information above from: UniProt accession P52630 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6773 Human
  • Entrez Gene: 20847 Mouse
  • Entrez Gene: 288774 Rat
  • Omim: 600556 Human
  • SwissProt: P52630 Human
  • SwissProt: Q9WVL2 Mouse
  • Unigene: 530595 Human
  • Unigene: 293120 Mouse

  • Unigene: 471333 Mouse
  • Unigene: 24237 Rat

  see all

• Alternative names
  Homo sapiens interferon alpha induced transcriptional activator antibodyinterferon alpha induced transcriptional activator antibodyISGF3 antibody

  MGC59816 antibodyP113 antibodysignal transducer and activator of transcription 2 113kD antibodySignal transducer and activator of transcription 2 antibodySTAT113 antibodyStat2 antibodySTAT2_HUMAN antibody

  see all

Anti-STAT2 (phospho Y690) antibody images

• 

  Western blot - STAT2 (phospho Y690) antibody (ab53132)
  All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution
  
  Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
  Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide
  
  
  Predicted band size : 98 kDa
  Observed band size : 98 kDa
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - STAT2 (phospho Y690) antibody (ab53132)
  ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.