Anti-STAT2 (phospho Y690) antibody (ab53132)

Overview

  • Product nameAnti-STAT2 (phospho Y690) antibodySee all STAT2 primary antibodies ...
  • Description
    Rabbit polyclonal to STAT2 (phospho Y690)
  • Specificityab53132 detects endogenous levels of STAT2 only when phosphorylated at tyrosine 690.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic phosphopeptide derived from human STAT2 around the phosphorylation site of tyrosine 690 (R-K-YP-L-K).

  • Positive control
    • WB: HeLa cell extracts IHC-P: Human lung carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab53132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/300 - 1/1000. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa).
IHC-P Use at an assay dependent dilution.
ELISA 1/20000.

Target

  • FunctionSignal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to IFN-alpha.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus upon activation by IFN-alpha/beta.
  • Target information above from: UniProt accession P52630 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Homo sapiens interferon alpha induced transcriptional activator antibody
    • interferon alpha induced transcriptional activator antibody
    • ISGF 3 antibody
    • ISGF3 antibody
    • MGC59816 antibody
    • P113 antibody
    • signal transducer and activator of transcription 2 113kD antibody
    • Signal transducer and activator of transcription 2 antibody
    • STAT113 antibody
    • Stat2 antibody
    • STAT2_HUMAN antibody
    see all

Anti-STAT2 (phospho Y690) antibody images

  • All lanes : Anti-STAT2 (phospho Y690) antibody (ab53132) at 1/300 dilution

    Lane 1 : HeLa cell extract treated with IFN (2500U/ml, 30mins)
    Lane 2 : HeLa cell extract treated with IFN (2500U/ml, 30mins) with immunising peptide


    Predicted band size : 98 kDa
    Observed band size : 98 kDa
  • ab53132, at 1/50 dilution, staining STAT2 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

References for Anti-STAT2 (phospho Y690) antibody (ab53132)

This product has been referenced in:
  • Shakya R  et al. Hypomethylating therapy in an aggressive stroma-rich model of pancreatic carcinoma. Cancer Res 73:885-96 (2013). Read more (PubMed: 23204224) »
  • Pattison MJ  et al. IFNß autocrine feedback is required to sustain TLR induced production of MCP-1 in macrophages. FEBS Lett 587:1496-503 (2013). WB ; Mouse . Read more (PubMed: 23542035) »

See all 6 Publications for this product

Product Wall

Unfortunately, the blocking peptide for ab53132 is not available. In order to assist in your determing what the identity of your two bands are, I did some research and found that IFN-alpha and IFN-beta induce activation of Jak kinases, which lead to ph...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citric acid (pH 6.0) for 10 min at ~100¯C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.02% triton
Fixative Paraformaldehyde
Username

Mr. Shachar Dagan

Verified customer

Submitted Mar 10 2014

Thank you fory our message which has been forwarded to me as my colleague ##### is away from the office today.

I am sorry I am not able to trace any previous correspondence from the email address you have used, and so I hope I can provide ad...

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Thank you for your enquiry.

Here is the link for the activated Stat1 reference we discussed:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3060772/?tool=pubmed

It uses the following antibody, ab4742:

http://www.abc...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab111609.


As we discussed, this antibody is backorder...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"