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Overview

  • Product nameAnti-STAT3 antibody [7G8]See all STAT3 primary antibodies ...
  • Description
    Mouse monoclonal [7G8] to STAT3
  • Tested applicationsSandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full-length Human STAT3 expressed in baculovirus

  • Positive controlHeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab69513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to STAT3 (ab15523). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to STAT3 (ab15523) as Detection.
WB WB: Use a concentration of 1 µg/ml. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).

Target

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.

    • Target information above from: UniProt accession P40763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      1110034C02Rik antibodyAcute Phase Response Factor antibodyAcute-phase response factor antibody
      APRF antibodyAW109958 antibodyDNA binding protein APRF antibodyFLJ20882 antibodyHIES antibodyMGC128731 antibodyMGC16063 antibodyMGC93551 antibodySignal transducer and activator of transcription 3 (acute-phase response factor) antibodySignal transducer and activator of transcription 3 antibodySignal Transductor and Activator of Transcription 3 antibodySTAT 3 antibodyStat3 antibodySTAT3_HUMAN antibodyTranscription factor antibody
    see all

Anti-STAT3 antibody [7G8] images

  • Sandwich ELISA - STAT3 antibody [7G8] (ab69513)
    Sandwich ELISA - STAT3 antibody [7G8] (ab69513)
    Standard Curve for STAT3 (Analyte: STAT3 protein (Tagged) (ab43618)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [7G8] to STAT3 (ab69513) at 5µg/ml and Detector Antibody Rabbit polyclonal to STAT3 (ab15523) at 0.5µg/ml.
  • Western blot - STAT3 antibody [7G8] (ab69513)
    Western blot - STAT3 antibody [7G8] (ab69513)
    All lanes : Anti-STAT3 antibody [7G8] (ab69513) at 1 µg/ml

    Lane 1 : MW markers
    Lane 2 : A431 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NIH-3T3 cell lysate

    developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 88 kDa

Protocols

References for Anti-STAT3 antibody [7G8] (ab69513)

This product has been referenced in:
  • Mukhopadhyay UK  et al. Doubles game: Src-Stat3 versus p53-PTEN in cellular migration and invasion. Mol Cell Biol 30:4980-95 (2010). Read more (PubMed: 20733006) »

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