Overview

  • Product nameAnti-STAT3 antibody [E121-21]See all STAT3 primary antibodies ...
  • Description
    Rabbit monoclonal [E121-21] to STAT3
  • SpecificityThe antibody only detects Stat3 without phosphorylation on Serine 727. It does not detect S727-phosphorylated Stat3.
  • Tested applicationsIHC-Fr, WB, IHC-P, IP more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow

    Does not react with

    Mouse, Rat
  • Immunogen

    A synthetic peptide corresponding to residues surrounding Ser727 of human Stat3.

  • Positive control
    • A431 cell lysate and lung squamous carcinoma.
  • General notes

    Produced under U.S. Patent No. 5,675,063. A 40 μl trial size is available to purchase for this antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32500 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-Fr Use at an assay dependent concentration. See Abreview.
WB 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 88 kDa).
IHC-P Use at an assay dependent dilution.
IP 1/20.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
    • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
    • Sequence similaritiesBelongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • Post-translational
      modifications
      Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
    • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
    • Target information above from: UniProt accession P40763 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • 1110034C02Rik antibody
      • Acute Phase Response Factor antibody
      • Acute-phase response factor antibody
      • APRF antibody
      • APRF antibody
      • AW109958 antibody
      • DNA binding protein APRF antibody
      • FLJ20882 antibody
      • HIES antibody
      • MGC16063 antibody
      • Signal transducer and activator of transcription 3 antibody
      • Signal Transductor and Activator of Transcription 3 antibody
      • Signal Transductor and Activator of Transcription 3 antibody
      • STAT 3 antibody
      • Stat3 antibody
      • STAT3_HUMAN antibody
      see all

    Anti-STAT3 antibody [E121-21] images

    • Anti-STAT3 antibody [E121-21] (ab32500) at 1/1000 dilution + A431 cell lysate

      Predicted band size : 88 kDa
      Observed band size : 92 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded lung squamous carcinoma using ab32500 at a 1/50 dilution.

    References for Anti-STAT3 antibody [E121-21] (ab32500)

    This product has been referenced in:

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (brain)
    Specification brain
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.05% Triton X-100/PBS
    Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 24 2009

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HEK cells)
    Specification HEK cells
    Fixative Methanol
    Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    Submitted Jun 19 2006

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Glia cell culture)
    Specification Glia cell culture
    Fixative Paraformaldehyde
    Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
    Username

    Dr. Randal Moldrich

    Verified customer

    Submitted Jun 19 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"