Overview

  • Product nameAnti-STAT3 antibody [STAAD22A]See all STAT3 primary antibodies ...
  • Description
    Mouse monoclonal [STAAD22A] to STAT3
  • Tested applicationsWB, ELISA, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to STAT3 aa 149-262.

  • Positive control
    • WB: NIH3T3 whole cell lysate and HeLa whole cell lysate. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
ELISA Use at an assay dependent concentration.
ICC 1/10.
Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 antibody
    • Signal Transductor and Activator of Transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 antibody [STAAD22A] images

  • Overlay histogram showing HeLa cells stained with ab50761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab50761, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-STAT3 antibody [STAAD22A] (ab50761) at 1/100 dilution

    Lane 1 : LPS treated rat brain
    Lane 2 : LPS treated rat brain
    Lane 3 : LPS treated rat brain
    Lane 4 : LPS treated rat brain
    Lane 5 : LPS treated rat brain
    Lane 6 : LPS treated rat brain
    Lane 7 : LPS treated rat brain
    Lane 8 : LPS treated rat brain
    Lane 9 : normal rat brain

    Lysates/proteins at 20 µg per lane.

    Secondary
    Lanes 1 - 8 : DAKO cytomation P0260 Conjugation: HRP at 1/3000 dilution
    Lane 9 : DAKO cytomation P0260 at 1/3000 dilution


    Predicted band size : 88 kDa

    This image is courtesy of an Abreview submitted by Dr Ruma Raha-Chowdhury

    Specific band at 88kDa in LPS treated brain (time course) probed with Abcam monoclonal STST 3 antibody (ab50761). Lane 1-8 LPS treated and lane 9 control brain sample

    See Abreview

  • Anti-STAT3 antibody [STAAD22A] (ab50761) at 1/100 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • HeLa cells labelled with ab50761 at 1/10 dilution, followed by Alexa Fluor 488 Goat anti-Mouse IgG at 1/200 dilution.
  • Anti-STAT3 antibody [STAAD22A] (ab50761) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution
    developed using the ECL technique

    Predicted band size : 88 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)

References for Anti-STAT3 antibody [STAAD22A] (ab50761)

This product has been referenced in:
  • Mazzone M  et al. Dose-dependent induction of distinct phenotypic responses to Notch pathway activation in mammary epithelial cells. Proc Natl Acad Sci U S A 107:5012-7 (2010). WB ; Human . Read more (PubMed: 20194747) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. This antibody will detect total STAT3. The immunogen sequence does not span any of the phosphorylation sites, so the antibody will detect both phosphorylated and unphosphorylated forms of STAT3. To our knowledge,...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (Brain)
Loading amount 20 µg
Specification Brain
Treatment LPS treated vs untreated
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Jun 02 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"