Overview

  • Product nameAnti-STAT3 antibodySee all STAT3 primary antibodies ...
  • Description
    Rabbit polyclonal to STAT3
  • Tested applicationsWB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    PMSPR

    , corresponding to amino acids 725-729 of Human STAT3.

  • Positive control
    • HeLa, HEK293T, Jurkat, 3T6, and THP1 cell lysates; recombinant Human STAT3 protein.
  • General notesFor storage at 4°C for several months add 0.1% NaN3.

Properties

Applications

Our Abpromise guarantee covers the use of ab126834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 88 kDa.
IP Use at 4 µg/mg of lysate.

Target

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • APRF antibody
    • AW109958 antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 antibody images

  • All lanes : Anti-STAT3 antibody (ab126834) at 1/1000 dilution

    Lane 1 : Human recombinant STAT1 beta
    Lane 2 : Human recombinant STAT2
    Lane 3 : Human recombinant STAT3
    Lane 4 : Human recombinant STAT4
    Lane 5 : Human recombinant STAT5
    Lane 6 : Human recombinant STAT6

    Lysates/proteins at 0.02 µg per lane.


    Predicted band size : 88 kDa
  • Detection of STAT3 by Western Blot of Immunprecipitate.
    ab126834, at a concentration of 4µg/mg lysate, staining STAT3 in HeLa (1), HEK293T (2), Jurkat (3), 3T6 (4), and THP1 (5) cell lysate by Immunoprecipitation.
  • All lanes : Anti-STAT3 antibody (ab126834) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK293T cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : THP1 cell lysate
    Lane 5 : 3T6 cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 88 kDa

References for Anti-STAT3 antibody (ab126834)

ab126834 has not yet been referenced specifically in any publications.

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