Overview

  • Product nameAnti-STAT3 antibodySee all STAT3 primary antibodies ...
  • Description
    Rabbit polyclonal to STAT3
  • Specificityab31370 detects endogenous levels of STAT3 protein around Tyrosine 705.
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic non-phosphopeptide derived from human STAT3 around the phosphorylation site of Tyrosine 705.

  • Positive control
    • IHC: breast carcinoma WB: K562 cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab31370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 88 kDa).
IHC-P Use at an assay dependent dilution.
ELISA 1/4000.

Target

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
  • Target information above from: UniProt accession P40763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • APRF antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 antibody
    • Signal Transductor and Activator of Transcription 3 antibody
    • Signal Transductor and Activator of Transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 antibody images

  • Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using anti-STAT3 antibody(ab31370, diluted 1:100); Right: The same antibody preincubated with synthesized peptide.
  • All lanes : Anti-STAT3 antibody (ab31370) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate with synthetic non-phosphopeptide


    Predicted band size : 88 kDa
    Observed band size : 95 kDa (why is the actual band size different from the predicted?)

References for Anti-STAT3 antibody (ab31370)

This product has been referenced in:
  • Tanaka T  et al. Melatonin suppresses AOM/DSS-induced large bowel oncogenesis in rats. Chem Biol Interact 177:128-36 (2009). IHC-P ; Rat . Read more (PubMed: 19028472) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. The treatment of PFA-fixed, paraffin embedded sections (IHC-P) before incubation with priamry antibody would include antigen retrieval, blocking of endogenous peroxidases (if HRP is being used for detection), and bl...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (adult brain Hippocampal region)
Specification adult brain Hippocampal region
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 29 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"