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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT3 antibody
See all STAT3 products (25) ...
Description
Rabbit polyclonal to STAT3
Specificity
ab31370 detects endogenous levels of STAT3 protein around Tyrosine 705.
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic non-phosphopeptide derived from human STAT3 around the phosphorylation site of Tyrosine 705.
Positive control
IHC: breast carcinoma WB: K562 cell extract
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Signal transduction >> Nuclear signaling >> STAT family
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 95 kDa (predicted molecular weight: 88 kDa).
IHC-P: 1/50 - 1/100.
ELISA
ELISA: 1/4000ELISA: 1/4000
Target
Function
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Tissue specificity
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease
Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
Target information above from: UniProt accessionP40763
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
see all
Database links
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P40763 Human
- SwissProt: P42227 Mouse
- SwissProt: P52631 Rat
- Unigene: 463059 Human
see all
Anti-STAT3 antibody images:
Immunohistochemistry (Paraffin-embedded sections) - STAT3 antibody (ab31370)
Immunohistochemical analysis of paraffin-embedded breast carcinoma. Left: Using anti-STAT3 antibody(ab31370, diluted 1:100); Right: The same antibody preincubated with synthesized peptide.
Western blot - STAT3 antibody (ab31370)
All lanes : Anti-STAT3 antibody (ab31370) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : K562 cell lysate with synthetic non-phosphopeptide
Predicted band size : 88 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
References for Anti-STAT3 antibody (ab31370)
This product has been referenced in:
- Tanaka Tet al. Melatonin suppresses AOM/DSS-induced large bowel oncogenesis in rats. Chem Biol Interact 177:128-36 (2009). IHC-P; Rat.Read more (PubMed: 19028472) »
See 1 publication for this product
Publishing research using ab31370? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"