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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab5073? Please let us know so that we can cite the reference in this datasheet
ab5073 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection using Goat polyclonal to STAT3 (ab5073) on rat (mixed neuron/glia) cell culture. Cells were fixed with paraformaldehyde, no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 1hr at room temperature (25 µg/ml). The confocal image shows anti-STAT3 positive immunoreactivity (red) in glial processes and nuclei. Blue nuclear counterstain is shown. Secondary antibody was donkey anti-goat IgG Alex fluor 568 (1/800).
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection of STAT3 using Goat polyclonal to STAT3 (ab5073) on human HEK cell culture. 5% BSA was used as a blocking agent for 40 mins. Cells were fixed with methanol; no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 12 hour at RT (1/100). The confocal image shows anti-STAT3 cytosolic and plasma membrane localisation as expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunohistochemistry (PFA perfusion fixed frozen sections) - STAT3 antibody (ab5073)
ab5073 at 10ug/ml staining STAT3 in mouse thymus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). The section incubated with biotinylated mouse anti goat as secondary, then Streptavidin-AP conjugate and developed using BCIP/NBT detection system.
Western blot - STAT3 antibody (ab5073)
Anti-STAT3 antibody (ab5073) at 1 µg/ml + NIH3T3 cell lysate in RIPA buffer at 35 µg
Predicted band size : 85 kDa
Primary incubation for 1 hour. Detected by chemiluminescence.
STAT3 antibody for ICC/IF in Human (5073)
STAT3 antibody for ICC/IF in Rat (5073)
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