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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection using Goat polyclonal to STAT3 (ab5073) on rat (mixed neuron/glia) cell culture. Cells were fixed with paraformaldehyde, no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 1hr at room temperature (25 µg/ml). The confocal image shows anti-STAT3 positive immunoreactivity (red) in glial processes and nuclei. Blue nuclear counterstain is shown. Secondary antibody was donkey anti-goat IgG Alex fluor 568 (1/800).
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection of STAT3 using Goat polyclonal to STAT3 (ab5073) on human HEK cell culture. 5% BSA was used as a blocking agent for 40 mins. Cells were fixed with methanol; no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 12 hour at RT (1/100). The confocal image shows anti-STAT3 cytosolic and plasma membrane localisation as expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunohistochemistry (PFA perfusion fixed frozen sections) - STAT3 antibody (ab5073)
ab5073 at 10ug/ml staining STAT3 in mouse thymus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). The section incubated with biotinylated mouse anti goat as secondary, then Streptavidin-AP conjugate and developed using BCIP/NBT detection system.
Western blot - STAT3 antibody (ab5073)
Anti-STAT3 antibody (ab5073) at 1 µg/ml + NIH3T3 cell lysate in RIPA buffer at 35 µg
Predicted band size : 85 kDa
Primary incubation for 1 hour. Detected by chemiluminescence.
STAT3 antibody for ICC/IF in Human (5073)
STAT3 antibody for ICC/IF in Rat (5073)
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