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Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
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Western blot - STAT3 antibody (ab5073)
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Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
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Product Name
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STAT3 antibody
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See all STAT3 antibodies (20)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to STAT3
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Immunogen
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Synthetic peptide: DMELTSECATSPM, corresponding to amino acids 758-770 of Human STAT 3.
BLAST 'DMELTSECATSPM' with  or 
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Reacts with
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Hu, Ms, Rat
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Tested applications
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ICC/IF, IHC-FoFr, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: Use at an assay dependent concentration.
IHC-FoFr: Use at a concentration of 10 µg/ml.
WB: Use at a concentration of 0.5 µg/ml. Detects a band of approximately 80-85 kDa (predicted molecular weight: 85 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic and Nuclear.
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Research areas
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Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cancer >> Signal transduction >> Nuclear signaling >> STAT family
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
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Relevance
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STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris-saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this STAT3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for STAT3 antibody images, references, products related to ab5073 and other tools.
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STAT3 antibody images:
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 Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection using Goat polyclonal to STAT3 (ab5073) on rat (mixed neuron/glia) cell culture. Cells were fixed with paraformaldehyde, no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 1hr at room temperature (25 µg/ml). The confocal image shows anti-STAT3 positive immunoreactivity (red) in glial processes and nuclei. Blue nuclear counterstain is shown. Secondary antibody was donkey anti-goat IgG Alex fluor 568 (1/800). Randal Moldrich, CNRS UMR7637, ESPCI, France
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Western blot - STAT3 antibody (ab5073)
Predicted band size : 85 kDa
Goat polyclonal to STAT 3, ab5073 with (0.5µg/ml) of A431 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence
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Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection of STAT3 using Goat polyclonal to STAT3 (ab5073) on human HEK cell culture. 5% BSA was used as a blocking agent for 40 mins. Cells were fixed with methanol; no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 12 hour at RT (1/100). The confocal image shows anti-STAT3 cytosolic and plasma membrane localisation as expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
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Immunohistochemistry (PFA perfusion fixed frozen sections) - STAT3 antibody (ab5073)
ab5073 at 10ug/ml staining STAT3 in mouse thymus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). The section incubated with biotinylated mouse anti goat as secondary, then Streptavidin-AP conjugate and developed using BCIP/NBT detection system.
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Search PubMed (MEDLINE) for references to STAT3
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STAT3 antibody - more information
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Customer reviews (feedback) regarding STAT3 antibody |
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STAT3 antibody - related products:
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