Overview

  • Product nameAnti-STAT3 antibody
    See all STAT3 primary antibodies
  • Description
    Goat polyclonal to STAT3
  • SpecificityThis antibody is expected to recognise isoforms 1 and 2 (as represented by NP_644805.1 and NP_003141.2 respectively)
  • Tested applicationsICC/IF, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Cow, Human
    Predicted to work with: Mouse, Rat, Chicken, Pig, Xenopus laevis
  • Immunogen

    Synthetic peptide within Human STAT3 aa 758-770 (C terminal). The exact sequence is proprietary.
    Sequence:

    DMELTSECATSPM


    Database link: NP_644805.1
    (Peptide available as ab23064)

  • Positive control
    • Rat (mixed neuron/glia) cell culture, mouse thymus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab5073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 80-85 kDa (predicted molecular weight: 85 kDa).Can be blocked with Human STAT3 peptide (ab23064).
ELISA Use at an assay dependent dilution.

Peptide ELISA

Target

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 antibody
    • Signal Transductor and Activator of Transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 antibody images

  • Immunofluorescence detection using Goat polyclonal to STAT3 (ab5073) on rat (mixed neuron/glia) cell culture. Cells were fixed with paraformaldehyde, no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 1hr at room temperature (25 µg/ml). The confocal image shows anti-STAT3 positive immunoreactivity (red) in glial processes and nuclei. Blue nuclear counterstain is shown. Secondary antibody was donkey anti-goat IgG Alex fluor 568 (1/800) ab175474).

  • Immunofluorescence detection of STAT3 using Goat polyclonal to STAT3 (ab5073) on human HEK cell culture. 5% BSA was used as a blocking agent for 40 mins. Cells were fixed with methanol; no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 12 hour at RT (1/100). The confocal image shows anti-STAT3 cytosolic and plasma membrane localisation as expected.

  • Anti-STAT3 antibody (ab5073) at 1 µg/ml + NIH3T3 cell lysate in RIPA buffer at 35 µg

    Predicted band size : 85 kDa
    Primary incubation for 1 hour. Detected by chemiluminescence.
  • ab5073 at 10ug/ml staining STAT3 in mouse thymus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). The section incubated with biotinylated mouse anti goat as secondary, then Streptavidin-AP conjugate and developed using  BCIP/NBT detection system.

  • ab5073 (4µg/ml) staining of paraffin embedded Mouse Thymus shows preferential staining of the medula over the cortex. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

References for Anti-STAT3 antibody (ab5073)

This product has been referenced in:
  • Chou CK  et al. mMAPS: a flow-proteometric technique to analyze protein-protein interactions in individual signaling complexes. Sci Signal 7:rs1 (2014). Mouse . Read more (PubMed: 24595109) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cell (brain stem)
Specification brain stem
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Dec 19 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK cells)
Specification HEK cells
Fixative Methanol
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

Submitted Jun 15 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (mixed neuron/glia cell culture)
Specification mixed neuron/glia cell culture
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 5%
Username

Dr. Randal Moldrich

Verified customer

Submitted Jun 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"