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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT3 antibody
See all STAT3 products (25) ...
Description
Goat polyclonal to STAT3
Tested applications
IHC-FoFr, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat, Cow, Dog
Immunogen
Synthetic peptide: DMELTSECATSPM, corresponding to amino acids 758-770 of Human STAT 3.
DMELTSECAT SPM
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Signal transduction >> Nuclear signaling >> STAT family
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use a concentration of 10 µg/ml
ICC/IF: Use at an assay dependent concentration.
WB: Use a concentration of 0.5 µg/mlDetects a band of approximately 80-85 kDa (predicted molecular weight: 85 kDa).
Target
Function
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Tissue specificity
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease
Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
Target information above from: UniProt accessionP40763
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
see all
Database links
- Entrez Gene: 508541 Cow
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P61635 Cow
- SwissProt: P40763 Human
- SwissProt: P42227 Mouse
see all
Anti-STAT3 antibody images:
Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection using Goat polyclonal to STAT3 (ab5073) on rat (mixed neuron/glia) cell culture. Cells were fixed with paraformaldehyde, no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 1hr at room temperature (25 µg/ml). The confocal image shows anti-STAT3 positive immunoreactivity (red) in glial processes and nuclei. Blue nuclear counterstain is shown. Secondary antibody was donkey anti-goat IgG Alex fluor 568 (1/800).
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunocytochemistry/ Immunofluorescence - STAT3 antibody (ab5073)
Immunofluorescence detection of STAT3 using Goat polyclonal to STAT3 (ab5073) on human HEK cell culture. 5% BSA was used as a blocking agent for 40 mins. Cells were fixed with methanol; no antigen retrieval step was necessary. Primary antibody ab5073 was incubated for 12 hour at RT (1/100). The confocal image shows anti-STAT3 cytosolic and plasma membrane localisation as expected.
Randal Moldrich, CNRS UMR7637, ESPCI, France
Immunohistochemistry (PFA perfusion fixed frozen sections) - STAT3 antibody (ab5073)
ab5073 at 10ug/ml staining STAT3 in mouse thymus tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). The section incubated with biotinylated mouse anti goat as secondary, then Streptavidin-AP conjugate and developed using BCIP/NBT detection system.
Western blot - STAT3 antibody (ab5073)
Anti-STAT3 antibody (ab5073) at 1 µg/ml + NIH3T3 cell lysate in RIPA buffer at 35 µg
Predicted band size : 85 kDa
Primary incubation for 1 hour. Detected by chemiluminescence.
References for Anti-STAT3 antibody (ab5073)
ab5073 has not yet been referenced specifically in any publications.
Publishing research using ab5073? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"