STAT3 antibody, prediluted (ab15524)

Product Name 

STAT3 antibody, prediluted

Product type 

Primary antibodies

Description 

Rabbit polyclonal to STAT3, prediluted

Immunogen 

Recombinant fragment: E SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY, corresponding to amino acids 50-240 of Human STAT3.
BLAST 'E SHATLVFH NL LGEIDQQ YSR FLQESN VLYQ HNLRR IKQFL QSRY LEKPME IAR IVARCLW EE SRLLQTAA TAAQQGGQAN HPTAAVVTE K QQMLEQHL QD VRKRVQD LEQ KMKVVE NLQD DFDFN YKTLK SQGD MQDLNG NNQ SVTRQKM QQ LEQMLTAL D QMRRSIVSE LAGLLSAMEY ' with or

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Chk, Cow
(Due to sequence homology)

Tested applications 
(see key)

IHC-P

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-P: Ready to use for 10 min at room temperature.
Staining of formalin fixed tissues requires boiling tissue sections in 10mM
citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Breast carcinoma.

Cellular localization 

Cytoplasmic and Nuclear.

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cancer >> Signal transduction >> Nuclear signaling >> STAT family
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

Relevance 

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Prediluted

Storage instructions 

Store at +4°C. Do not freeze.

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