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Anti-STAT3 antibody, prediluted (ab15524)

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Overview

Product name

Anti-STAT3 antibody, prediluted
See all STAT3 products (25) ...

Description

Rabbit polyclonal to STAT3, prediluted

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Xenopus laevis

Immunogen

Recombinant fragment: E SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRRIKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQDLEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQSVTRQKM QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY, corresponding to amino acids 50-240 of Human STAT3.

E SHATLVFHNL LGEIDQQYSR FLQESNVLYQ HNLRR IKQFL QSRYLEKPME IARIVARCLW EESRLLQTAA TAAQQGGQAN HPTAAVVTEK QQMLEQHLQD VRKRVQD LEQ KMKVVENLQD DFDFNYKTLK SQGDMQDLNG NNQ SVTRQKM QQLEQMLTAL DQMRRSIVSE LAGLLSAMEY

Positive control

Breast carcinoma.

Properties

Form

Prediluted

Storage instructions

Store at +4°C. Do not freeze.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 antibody, prediluted (ab15524)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 antibody, prediluted (ab15524) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Ready to use for 10 min at room temperature.
Staining of formalin fixed tissues requires boiling tissue sections in 10mM
citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.

Tissue specificity

Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.

Involvement in disease

Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.

Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

Post-translational
modifications

Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.

Cellular localization

Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.

Target information above from: UniProt accessionP40763 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 1110034C02Rik antibody
  • Acute Phase Response Factor antibody
  • Acute-phase response factor antibody
  • APRF antibody
  • AW109958 antibody
  • DNA binding protein APRF antibody
  • FLJ20882 antibody
  • HIES antibody
  • MGC128731 antibody
  • MGC16063 antibody
  • MGC93551 antibody
  • Signal transducer and activator of transcription 3 (acute-phase response factor) antibody
  • Signal transducer and activator of transcription 3 antibody
  • Signal Transductor and Activator of Transcription 3 antibody
  • STAT 3 antibody
  • Stat3 antibody
  • STAT3_HUMAN antibody
  • Transcription factor antibody
see all

Anti-STAT3 antibody, prediluted images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 antibody, prediluted (ab15524)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 antibody, prediluted (ab15524)

ab15524 staining STAT3 in Hodgkin's lymphoma by Immunohistology (formalin/paraffin).

References for Anti-STAT3 antibody, prediluted (ab15524)

ab15524 has not yet been referenced specifically in any publications.

Publishing research using ab15524? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"