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Overview

  • Product nameSTAT3 (pY705) ELISA KitSee all STAT3 (phospho-Tyr705) kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell Lysate
  • Assay typeSandwich
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Product overview

    ab126458 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated STAT3 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-STAT3 (Tyr705). An anti-pan STAT3 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and STAT3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-STAT3 (Tyr705) antibody is used to detect phosphorylated STAT3 (Tyr705). After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of STAT3 (Tyr705) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.

    • Target information above from: UniProt accession P40763 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Acute-phase response factorAPRFDNA binding protein APRF
        HIESSignal transducer and activator of transcription 3Stat3STAT3_HUMAN
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab126458 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      STAT3 (pY705) ELISA Kit images

      • Sandwich ELISA - STAT3 (pY705) ELISA Kit (ab126458)
        Sandwich ELISA - STAT3 (pY705) ELISA Kit (ab126458)
        A431 cells were treated with recombinant human EGF at 37°C for 20 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
      • Sandwich ELISA - STAT3 (pY705) ELISA Kit (ab126458)
        Sandwich ELISA - STAT3 (pY705) ELISA Kit (ab126458)
        A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 20 min. Cell lysates were analyzed using this phosphoELISA.
      • Western blot - STAT3 (pY705) ELISA Kit (ab126458)
        Western blot - STAT3 (pY705) ELISA Kit (ab126458)
        A431 cells were treated or untreated with 100 ng/ml recombinant human EGF for 20 min. Cell lysates were analyzed using Western Blot.
      • Sandwich ELISA - STAT3 (pY705) ELISA Kit (ab126458)
        Sandwich ELISA - STAT3 (pY705) ELISA Kit (ab126458)
        The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes. Serial dilutions of lysates were analyzed in this ELISA.
      • Western blot - STAT3 (pY705) ELISA Kit (ab126458)
        Western blot - STAT3 (pY705) ELISA Kit (ab126458)
        The A431 cells were treated with 100 ng/ml recombinant human EGF for 20 minutes. Serial dilutions of lysates were analyzed by Western blot. Immunoblots were incubated with anti-phospho-STAT3 (Tyr705).

      Protocols

      References for STAT3 (pY705) ELISA Kit (ab126458)

      ab126458 has not yet been referenced specifically in any publications.

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