Anti-STAT3 (phospho S727) antibody (ab30647)

Overview

  • Product nameAnti-STAT3 (phospho S727) antibody
    See all STAT3 primary antibodies
  • Description
    Rabbit polyclonal to STAT3 (phospho S727)
  • Specificityab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
  • Tested applicationsIHC-FoFr, IHC-P, WB, ELISA, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    Synthetic peptide corresponding to Human STAT3 (propionyl S727). The antiserum was produced against synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of serine 727 (P-M-SP-P-R).
    Database link: P40763

  • Positive control
    • IHC: breast carcinoma WB: HeLa cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab30647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent dilution. PubMed: 20950279
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).Can be blocked with Human STAT3 full length protein (ab43618) or Human STAT3 full length protein (ab82053).
ELISA 1/10000.
IP Use at an assay dependent dilution.

Target

  • FunctionTranscription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
  • Tissue specificityHeart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Involvement in diseaseDefects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
  • Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110034C02Rik antibody
    • Acute Phase Response Factor antibody
    • Acute-phase response factor antibody
    • APRF antibody
    • APRF antibody
    • AW109958 antibody
    • DNA binding protein APRF antibody
    • FLJ20882 antibody
    • HIES antibody
    • MGC16063 antibody
    • Signal transducer and activator of transcription 3 antibody
    • Signal Transductor and Activator of Transcription 3 antibody
    • Signal Transductor and Activator of Transcription 3 antibody
    • STAT 3 antibody
    • Stat3 antibody
    • STAT3_HUMAN antibody
    see all

Anti-STAT3 (phospho S727) antibody images

  • All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)

    Lane 1 : HeLa cells extract
    Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
    Lane 3 : HeLa cells extract with Synthesized phosphopeptide


    Predicted band size : 88 kDa
    Observed band size : 88 kDa
  • ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.

References for Anti-STAT3 (phospho S727) antibody (ab30647)

This product has been referenced in:
  • Xu B  et al. Menin promotes hepatocellular carcinogenesis and epigenetically up-regulates Yap1 transcription. Proc Natl Acad Sci U S A 110:17480-5 (2013). WB ; Mouse . Read more (PubMed: 24101467) »
  • Sutherland JM  et al. Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation. J Cell Physiol 227:1188-98 (2012). WB, IHC-P ; Mouse . Read more (PubMed: 21604262) »

See all 7 Publications for this product

Product Wall

Application Western blot
Loading amount 45 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Lung)
Specification Lung
Treatment Basel conditions
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Oct 22 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% TritonX in 0.1% PBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted May 11 2012

Thank you for your inquiry.

I strongly suggest to use a pre-absorbed secondary antibody so that the cross-binding to the two rat monoclonals can be minimized.

Since you are already using fluorescence in the green and red spectrum,...

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