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Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Overview
Product name
Anti-STAT3 (phospho S727) antibody
See all STAT3 products (25) ...
Description
Rabbit polyclonal to STAT3 (phospho S727)
Specificity
ab30647 detects endogenous levels of STAT3 only when phosphorylated at Serine 727.
Tested applications
IHC-FoFr, IHC-P, WB, ELISA, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of Serine 727.
Positive control
IHC: breast carcinoma WB: HeLa cells lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg++ and Ca++), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab30647 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cardiovascular >> Heart >> Hypertrophy >> Transcription factors
Cardiovascular >> Heart >> Cardiogenesis >> Transcription factors/regulators
Cancer >> Signal transduction >> Nuclear signaling >> STAT family
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 20950279)
IHC-P: 1/50 - 1/100.Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
ELISA
ELISA: 1/10000ELISA: 1/10000
IP: Use at an assay dependent dilution.
Target
Function
Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
Tissue specificity
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Involvement in disease
Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.
Post-translational
modifications
Tyrosine phosphorylated upon stimulation with EGF (By similarity). Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus.
Cellular localization
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation. Predominantly present in the cytoplasm without stimuli. Upon leukemia inhibitory factor (LIF) stimulation, accumulates in the nucleus. The complex composed of BART and ARL2 plays an important role in the nuclear translocation and retention of STAT3.
Target information above from: UniProt accessionP40763
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 1110034C02Rik antibody
- Acute Phase Response Factor antibody
- Acute-phase response factor antibody
see all
Database links
- Entrez Gene: 6774 Human
- Entrez Gene: 20848 Mouse
- Entrez Gene: 25125 Rat
- Omim: 102582 Human
- SwissProt: P40763 Human
- SwissProt: P42227 Mouse
- SwissProt: P52631 Rat
- Unigene: 463059 Human
see all
Anti-STAT3 (phospho S727) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STAT3 (phospho S727) antibody (ab30647)
ab30647 at 1/50 dilution staining STAT3 (phospho S727) in human breast carcinoma tissue by Immunohistochemistry (Formalin/PFA fixed paraffin embedded sections). The left image show staining by ab30647 without specific peptide while right image show staining after blocking with peptide.
Western blot - STAT3 (phospho S727) antibody (ab30647)
All lanes : Anti-STAT3 (phospho S727) antibody (ab30647)
Lane 1 : HeLa cells extract
Lane 2 : HeLa cells extract with Synthesized non-phosphopeptide
Lane 3 : HeLa cells extract with Synthesized phosphopeptide
Predicted band size : 88 kDa
Observed band size : 88 kDa
References for Anti-STAT3 (phospho S727) antibody (ab30647)
This product has been referenced in:
- Ramasamy Ket al. Silibinin prevents lung tumorigenesis in wild-type but not in iNOS-/- mice: potential of real-time micro-CT in lung cancer chemoprevention studies. Clin Cancer Res 17:753-61 (2011). IHC-P; Mouse.Read more (PubMed: 21148748) »
- Brandt MDet al. Physical exercise increases Notch activity, proliferation and cell cycle exit of type-3 progenitor cells in adult hippocampal neurogenesis. Eur J Neurosci 32:1256-64 (2010). IHC-FoFr; Mouse.Read more (PubMed: 20950279) »
See all 4 publications for this product
Publishing research using ab30647? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"