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ab171358 is a PBS-only buffer formulated version of ab76315, containing not BSA or sodium azide, ideal for antibody labeling. Please refer to ab76315 for information on protocols, dilutions, and image data.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab171358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).
|WB||Use at an assay dependent concentration. Predicted molecular weight: 88 kDa.|
This IHC data was generated using the same anti-phospho STAT3 Y705 antibody clone, EP2147Y, in a different buffer formulation (cat# ab76315).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling STAT3 (phospho Y705) with unpurified ab76315 at 1/100.
This ICC/IF data was generated using the same anti-phospho STAT3 Y705 antibody clone, EP2147Y, in a different buffer formulation (cat# ab76315).
Immunocytochemistry/Immunofluorescence analysis of HeLa +/- IFN-α (50ng/mL, 5 minutes) cells labelling STAT3 (phospho Y705) with ab76315 at 1/500 (4.3 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1500) was used as the secondary antibody. DAPI (blue) was used as a nuclear counterstain.
ab171358 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"