Western blot abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (peripheral blood CD19+ B cells)
Loading amount
200 µg
Specification
peripheral blood CD19+ B cells
Treatment
(1) Untreated (2) 100 ng recombinant hIL-10 for 24hours
Gel Running Conditions
Reduced Denaturing (10 % SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % milk blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP conjugated anitbody (Sant
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 86 kDa Non-specific: 78 kDa
Positive control
Peripheral blood CD19+ B cells treated with recombinant human IL-10 for 24 hours.
Negative control
Untreated B cells.

Additional data

Additional Notes
Excellent antibody that works extremely well. Long exposure of films will reveal non-specific bands however the antibody works so well that this type of long exposure is simply not necessary.
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Submitted Jul 07 2010

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