Western blot abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Lung)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
22 µg
Specification
Lung
Blocking step
Odyssey TBS Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C

Other product details

Incubation time
6 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 50% Odyssey TBS Block with 0.2% Tween-20
Dilution
1/100000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: anti-rabbit IgG, 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
Near-infrared
Negative control
Not included.
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 92 kDa
Positive control
Band of interest overlapped with total STAT-3 antibody, which was used in the 700 channel (not shown).
Username

Pete Serbedzija

Verified customer

Submitted Jul 28 2016

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