STAT3 (phospho Y705) antibody (ab30646)

Product Name 

STAT3 (phospho Y705) antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to STAT3 (phospho Y705)

Immunogen 

Synthesized phosphopeptide derived from human STAT3 around the phosphorylation site of Tyrosine 705.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab30646 detects endogenous levels of STAT3 only when phosphorylated at Tyrosine 705.

Tested applications 
(see key)

ELISA, IHC-Fr, IHC-P, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/20000.
IHC-P: 1/50 - 1/100.
IP: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

IHC: breast carcinoma
WB: HeLa cells treated with INF alpha

Cellular localization 

Cytoplasmic and Nuclear.

Research areas 

Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Cancer >> Signal transduction >> Nuclear signaling >> STAT family
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> STATs
Stem Cells >> Embryonic Stem Cells >> Intracellular
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

Relevance 

STATs (signal transducers and activators of transcription) were originally discovered as two proteins (STAT1 and STAT2) which were involved in interferon alpha (IFN alpha) and IFN gamma signal transduction. Since then, several additional STAT proteins have been identified (STAT3, 4, 5a, 5b, and 6). STATs undergo tyrosine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. In some cases this process is mediated by JAK Kinases (Janus Kinases 1, 2, and 3). For maximum activation of these proteins, phosphorylation at specific tyrosine and serine residues may be required in STAT1 alpha, 3, 4, and 5.
Specific functions of the various members of the STAT family are poorly understood. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
STAT1 and STAT2 are induced by IFN alpha and forn 1, a heterodimer which is part of the ISGF transcription factor complex. The STAT3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFNa, IL10, IL6, IL11, IL12, IL2, EGF, among others. STAT3 beta appears to be activated by both EGF and IL6, but STAT3 alpha is only activated by EGF. STAT3 binds to DNA as either a homodimer or as a heterodimer with STAT1. STAT3 interacts with the APRE acute phase response element) and SIE (csis inducible element). STAT4, predominantly expressed in testis and myeloid cells, is activated by ILl2. STAT5 is activated by prolactin and IL3. STAT6 participates in signaling pathways mediated by IL4.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg⁺⁺ and Ca⁺⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for STAT3 (phospho Y705) antibody images, references, products related to ab30646 and other tools.

STAT3 (phospho Y705) antibody images:

References for STAT3 (phospho Y705) antibody (ab30646)

  This product has been used in: (two most recent references)

Tutter AV et al. Role for Med12 in regulation of Nanog and Nanog target genes. J Biol Chem 284:3709-18 (2009). WB; Mouse. PubMed: 19036726

Xiao H et al. The Toll-interleukin-1 receptor member SIGIRR regulates colonic epithelial homeostasis, inflammation, and tumorigenesis. Immunity 26:461-75 (2007). PubMed: 17398123
If you publish research using ab30646 please let us know so that we can cite the reference on this datasheet.

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