Anti-STAT4 (phospho Y693) antibody (ab28815)
Key features and details
- Rabbit polyclonal to STAT4 (phospho Y693)
- Suitable for: IP, WB, IHC-P, ELISA
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-STAT4 (phospho Y693) antibody
See all STAT4 primary antibodies -
Description
Rabbit polyclonal to STAT4 (phospho Y693) -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human STAT4 (phospho Y693). Human STAT4 around the phosphorylation site of Tyr693.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28815 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Predicted molecular weight: 85 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 85 kDa. |
IHC-P
1/50 - 1/100. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Involved in IL12 signaling. -
Involvement in disease
Systemic lupus erythematosus 11
Rheumatoid arthritis -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6775 Human
- Entrez Gene: 20849 Mouse
- Omim: 600558 Human
- SwissProt: Q14765 Human
- SwissProt: P42228 Mouse
- Unigene: 735572 Human
- Unigene: 80642 Human
- Unigene: 1550 Mouse
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Alternative names
- Signal transducer and activator of transcription 4 antibody
- SLEB11 antibody
- STAT4 antibody
- STAT4_HUMAN antibody
Images
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ab28815, staining human STAT4 in breast carcinoma by immunohistochemistry, paraffin embedded tissue. left: Using STAT4 antibody (ab28815); right: The same antibody preincubated with synthesized phosphopeptide.
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All lanes : Anti-STAT4 (phospho Y693) antibody (ab28815)
Lane 1 : HeLa cell lysate (cells treated with IL4)
Lane 2 : HeLa cell lysate (untreated)
Predicted band size: 85 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab28815 has been referenced in 10 publications.
- Xu S et al. Leukemia inhibitory factor is a therapeutic target for renal interstitial fibrosis. EBioMedicine 86:104312 (2022). PubMed: 36335669
- Yue X et al. Intracerebral Hemorrhage Induced Brain Injury Is Mediated by the Interleukin-12 Receptor in Rats. Neuropsychiatr Dis Treat 16:891-900 (2020). PubMed: 32308392
- Liu P et al. Baicalin suppresses Th1 and Th17 responses and promotes Treg response to ameliorate sepsis-associated pancreatic injury via the RhoA-ROCK pathway. Int Immunopharmacol 86:106685 (2020). PubMed: 32570032
- Xu K et al. Adoptive transfer of bone marrow-derived dendritic cells (BMDCs) alleviates OVA-induced allergic airway inflammation in asthmatic mice. Sci Rep 10:13915 (2020). PubMed: 32807859
- Wang Y et al. A lipid-soluble extract of Pinellia pedatisecta Schott enhances antitumor T cell responses by restoring tumor-associated dendritic cell activation and maturation. J Ethnopharmacol 241:111980 (2019). PubMed: 31146000
- Zuo L et al. Bryostatin-1 ameliorated experimental colitis in Il-10-/- Mice by protecting the intestinal barrier and limiting immune dysfunction. J Cell Mol Med 23:5588-5599 (2019). PubMed: 31251471
- Zhang CJ et al. Act1 is a negative regulator in T and B cells via direct inhibition of STAT3. Nat Commun 9:2745 (2018). PubMed: 30013031
- Chuang Y et al. Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immun 22:647-657 (2016). WB ; Mouse . PubMed: 27670945
- Cichocki F et al. Transcriptional regulation of Munc13-4 expression in cytotoxic lymphocytes is disrupted by an intronic mutation associated with a primary immunodeficiency. J Exp Med 211:1079-91 (2014). PubMed: 24842371
- Surls J et al. Increased Membrane Cholesterol in Lymphocytes Diverts T-Cells toward an Inflammatory Response. PLoS One 7:e38733 (2012). WB ; Mouse . PubMed: 22723880