Products:Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs
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ab8004 |
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ab7969 has been referenced in 4 publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
ab7969 staining mouse skin epidermis whole tissue lysate by X-ChIP. The cross linking step was 10 seconds with 1% formaldehyde. The primary antibody was diluted 1/180 and incubated for 12 hours at 4°C.
ChIP dilution buffer: 16.7mm tris-cl (pH- 8.1) 0.01% SDS 1.1% triton-X-100 1.2mm EDTA, 16.7mm Nacl +1X complete protease inhibtor cocktail.
In the image, wild type (control) and TPA treated samples were immmunoprecipitated with rabbit IgG, pan RAR antibody or STAT5 antibody and the purified DNA was amplified using primers flanking the STAT5 binding element. Rabbit IgG and 2 other unrelated antibodies did not give any amplification.
PCR condition: 94, 30' 59, 30' 72, 45' 32 cycles. product size:310bp.
This image is courtesy of an anonymous Abreview
All lanes : Anti-STAT5 antibody - ChIP Grade (ab7969) at 1/1000 dilution
Lane 1 : Human Cell lysate - whole cell (colon cancer)
at 40 µg
Lane 2 : Human Cell lysate - whole cell (colon cancer)
at 80 µg
Secondary
Goat anti-rabbit IgG antibody
Conjugation: HRP
Performed under non-reducing conditions.
Predicted band size : 90 kDa
Observed band size : 90 kDa
This image is courtesy of an anonymous Abreview
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