Recombinant Anti-STAT5 (phospho Y694) antibody [E208] (ab32364)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E208] to STAT5 (phospho Y694)
- Suitable for: WB, IHC-P, ELISA
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-STAT5 (phospho Y694) antibody [E208]
See all STAT5 primary antibodies -
Description
Rabbit monoclonal [E208] to STAT5 (phospho Y694) -
Host species
Rabbit -
Specificity
This reagent is not recommended for mouse IHC-P
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Tested applications
Suitable for: WB, IHC-P, ELISAmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 cell lysate treated with epidermal growth factor. IHC-P: Human breast carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E208 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
|
IHC-P | (5) |
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Unsuitable for mouse. |
ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Unsuitable for mouse. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 6777 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 20851 Mouse
- Omim: 601511 Human
- Omim: 604260 Human
- SwissProt: P42229 Human
- SwissProt: P51692 Human
see all -
Alternative names
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
- Signal Transducer and Activator of Transcription 5B antibody
see all
Images
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All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (purified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with Epidermal growth factor
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 90 kDa
Observed band size: 90 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human infiltrating duct carcinoma of breast tissue labelling STAT5 (phospho Y694) with purified ab32364 at 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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Serially diluted ab32364 was bound to immobilised phospho peptide (P) - or control peptides ((C), 1 microgram x mL-1). The antibody was detected by HRP-labelled goat anti-rabbit IgG (ab97080; diluted 1: 50,000) and signal was developed with TMB substrate
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All lanes : Anti-STAT5 (phospho Y694) antibody [E208] (ab32364) at 1/1000 dilution (unpurified)
Lane 1 : A431 cell lysate, untreated
Lane 2 : A431 cell lysate, treated with EGF
Lane 3 : A431 cell lysate, treated with phosphatase
Predicted band size: 90 kDa
Observed band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma using unpurified ab32364 at a 1/50 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (59)
ab32364 has been referenced in 59 publications.
- Wu S et al. Interplay between hypertriglyceridemia and acute promyelocytic leukemia mediated by the cooperation of peroxisome proliferator-activated receptor-α with the PML/RAR α fusion protein on super-enhancers. Haematologica 107:2589-2600 (2022). PubMed: 35546300
- Schwarz M et al. Disrupting PTPRJ transmembrane-mediated oligomerization counteracts oncogenic receptor tyrosine kinase FLT3 ITD. Front Oncol 12:1017947 (2022). PubMed: 36452504
- Gao H et al. Characterization of murine mammary stem/progenitor cells in a D-galactose-induced aging model. Aging (Albany NY) 13:11762-11773 (2021). PubMed: 33878032
- Li R et al. Identification and In Vitro Functional Verification of Two Novel Mutations of GHR Gene in the Chinese Children with Laron Syndrome. Front Endocrinol (Lausanne) 12:605736 (2021). PubMed: 33912130
- Mäkinen A et al. IGF2BP3 Associates with Proliferative Phenotype and Prognostic Features in B-Cell Acute Lymphoblastic Leukemia. Cancers (Basel) 13:N/A (2021). PubMed: 33805930