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Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution + A431 cell lysate
Predicted band size : 91 kDa
Observed band size : 92 kDa (why is the actual band size different from the predicted?)
ab32043 at 1/250 dilution, staining human squamous lung carcinoma by Immunohistochemistry, paraffin-embedded tissue.
Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution +
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 20 seconds
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