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Anti-STAT5a antibody [E289] (ab32043)

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Overview

Product name

Anti-STAT5a antibody [E289]
See all STAT5a products (19) ...

Description

Rabbit monoclonal [E289] to STAT5a

Specificity

The antibody recognises Stat5a. It does not cross-react with other Stat family members.

Tested applications

WB, IHC-P, ICC/IF, Flow Cyt, IPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in C-terminus of human Stat5a.

Positive control

A431 cell lysate, human squamous lung carcinoma.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4

Concentration

Concentration information loading...

Purity

IgG fraction

Clonality

Monoclonal

Clone number

E289

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> STATs

  • Western blot - STAT5a antibody [E289] (ab32043)Western blot - STAT5a antibody [E289] (ab32043) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody [E289] (ab32043)Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody [E289] (ab32043) image (enlarge)

  • Western blot Western blot image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide2 Images

    WB

     WB: 1/1000Detects a band...Read more →

    WB: 1/1000Detects a band of approximately 92 kDa (predicted molecular weight: 91 kDa).

  • 1 Image

    IHC-P

     IHC-P: 1/100 - 1/250.

    IHC-P: 1/100 - 1/250.

  • ICC/IF

     ICC/IF: 1/100

    ICC/IF: 1/100

  • Flow Cyt

     Flow Cyt: 1/10

    Flow Cyt: 1/10

  • IP

     IP: 1/80

    IP: 1/80

Target

Function

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Sequence similarities

Belongs to the transcription factor STAT family.
Contains 1 SH2 domain.

Post-translational
modifications

Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.

Cellular localization

Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.

Target information above from: UniProt accessionP42229 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Mammary gland factor antibody
  • Mammary gland factor antibody
  • MGF antibody
  • MGF antibody
  • Signal transducer and activator of transcription 5A antibody
  • Signal transducer and activator of transcription 5A antibody
  • STA5A_HUMAN antibody
  • STAT 5 antibody
  • STAT 5A antibody
  • STAT5 antibody
  • STAT5 antibody
  • STAT5A antibody
see all

Database links

see all

Anti-STAT5a antibody [E289] images:

  Western blot - STAT5a antibody [E289] (ab32043)

Western blot - STAT5a antibody [E289] (ab32043)

Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution + A431 cell lysate

Predicted band size : 91 kDa
Observed band size : 92 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody [E289] (ab32043)

Immunohistochemistry (Paraffin-embedded sections) - STAT5a antibody [E289] (ab32043)

ab32043 at 1/250 dilution, staining human squamous lung carcinoma by Immunohistochemistry, paraffin-embedded tissue.

  Western blot

Western blot

Anti-STAT5a antibody [E289] (ab32043) at 1/1000 dilution + STAT5a protein (His tag) (ab84627) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 20 seconds

References for Anti-STAT5a antibody [E289] (ab32043)

ab32043 has not yet been referenced specifically in any publications.

Publishing research using ab32043? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"